The aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain reaction (PCR) As the reader moves through the book, the scope and complexity of each technique gradually increases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells.
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From the reviews:
"Single cell diagnostics has become an increasingly important field for both clinicians and researchers who are involved in infertility treatment. ... For researchers this book provides not only detailed state of the art protocols for single cell diagnostics, but also valuable notes on troubleshooting and pitfalls. For clinicians in the field of infertility this nice overview gives an insight into single cell diagnostics and its scientific background. ... This book can be warmly recommended to clinicians and researchers caring for patients with fertility problems." (G. Haidl and J. P. Allam, Andrologia, Vol. 40 (1), 2008)
"This book details innovative methods for analyzing gene expression, chromosome organization, and proteins in single cells and cell extracts. ... The book is written for basic science and clinical researchers, particularly those providing care to couples seeking advice regarding infertility and inherited genetic disorders. ... The chapters are written by experts and the book is carefully edited. ... The laboratory protocols and tips will be valued by laboratory scientists interested in forensic medicine, reproductive medicine, and developmental genetics." (Bruce A. Fenderson, Doody’s Review Service, October, 2007)
This book applies modern molecular diagnostic techniques to the analysis of single cells, small numbers of cells, or cell extracts. Emphasis is placed on non-invasive analysis of single cell metabolites and the direct analysis of RNA and DNA from single cells, with a focus on polymerase chain reaction and fluorescence in situ hybridization. In particular, this handbook is essential for practitioners providing care for couples seeking treatment for infertility.
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Taschenbuch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book applies modern molecular diagnostic techniques to the analysis of single cells, small numbers of cells, or cell extracts. Emphasis is placed on non-invasive analysis of single cell metabolites and the direct analysis of RNA and DNA from single cells, with a focus on polymerase chain reaction and fluorescence in situ hybridization. In particular, this handbook is essential for practitioners providing care for couples seeking treatment for infertility. 182 pp. Englisch. Nº de ref. del artículo: 9781617376542
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Condición: New. Editor(s): Thornhill, Alan, Ph.D (London Fertility Centre, London, UK). Num Pages: 182 pages, 10 black & white tables, biography. BIC Classification: MFGC; MFN; PSF. Category: (P) Professional & Vocational. Dimension: 229 x 152 x 11. Weight in Grams: 296. . 2010. 1st ed. Softcover of orig. ed. 2007. Paperback. . . . . Nº de ref. del artículo: V9781617376542
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Condición: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Up-to-date, clinically applicable, validated protocols for single-cell diagnosticsWhole genome amplification to create starting material from single cellsMicroarrays for analyzing multiple gene sequences simultaneously from a single cell. Nº de ref. del artículo: 458521512
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Taschenbuch. Condición: Neu. Single Cell Diagnostics | Methods and Protocols | Alan Thornhill | Taschenbuch | xi | Englisch | 2010 | Springer Nature B.V. | EAN 9781617376542 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu. Nº de ref. del artículo: 107146436
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