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9781607616627: Microarray Methods for Drug Discovery: 632 (Methods in Molecular Biology, 632)

Sinopsis

1. Multicenter Clinical Sample Collection for Microarray AnalysisTony S. Mondala, Daniel R. Salomon, and Steven R. Head2. Selective Isolation of Total RNA from Mouse Melanoma Subsets Using Fluorescence Activated Cell SortingScott Tighe and Matthew A. Held3. Microarray Analysis of Embryonic Stem Cells and Differentiated Embryoid BodiesAlexander C. Zambon and Christopher S. Barker4. Determination of Alternate Splicing Events Using the Affymetrix Exon 1.0 ST ArraysSita Subbaram, Marcy Kuentzel, David Frank, C. Michael DiPersio, and Sridar V. Chittur5. Profiling microRNA Expression with the Illumina BeadChip PlatformJulissa Tsao, Patrick Yau, and Neil Winegarden6. TaqMan® Array Cards in Pharmaceutical ResearchDavid N. Keys, Janice K. Au-Young, and Richard A. Fekete7. DMET™ Microarray Technology for Pharmacogenomics-Based Personalized MedicineJames K. Burmester, Marina Sedova, Michael H. Shapero, and Elaine Mansfield8. The Use of Microarray Technology for CytogeneticsBassem A. Bejjani, Lisa G. Shaffer, and Blake C. Ballif9. PCR/LDR/Universal Array Platforms for the Diagnosis of Infectious DiseaseManeesh Pingle, Mark Rundell, Sanchita Das, Linnie M. Golightly, and Francis Barany10. RIP-CHIP in Drug DevelopmentRitu Jain, Francis Doyle, Ajish D. George, Marcy Kuentzel, David Frank, Sridar V. Chittur, and Scott A. Tenenbaum11. ChIPing Away At Global Transcriptional RegulationKelly Jackson, James Paris, and Mark Takahashi12. HELP (HpaII Tiny Fragment Enrichment by Ligation-Mediated PCR) Assay for DNA Methylation Profiling of Primary Normal and Malignant B LymphocytesRita Shaknovich, Maria E. Figueroa, and Ari Melnick13. High-Throughput Screening of Metalloproteases Using SmallMolecule MicroarraysMahesh Uttamchandani14. Metabolic Enzyme Microarray Coupled with Miniaturized Cell-Culture Array Technology for High-Throughput Toxicity ScreeningMoo-Yeal Lee, Jonathan S. Dordick, and Douglas S. Clark15. Use of Tissue Microarray to Facilitate Oncology ResearchPanagiotis Gouveris, Paul M. Weinberger, and Amanda Psyrri16. Small Molecule Selectivity and Specificity Profiling Using Functional Protein MicroarraysPeter R. Kraus, Lihao Meng, and Lisa Freeman-Cook17. Production and Application of Glycan MicroarraysJulia Busch, Ryan McBride, and Steven R. Head

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De la contraportada

While the utilization of microarrays for gene expression studies has been widely published, Microarray Methods for Drug Discovery describes the use of this technology for a multitude of other vital applications. Covering the field of drug discovery through its various aspects, including high throughput screening, target identification, drug metabolism and toxicity screening as well as clinical sample handling, this volume also addresses new emerging fields like miRNA profiling, ribonomic and glycomic profiling. All chapters, contributed by experts in the field, follow the highly successful Methods in Molecular Biology™ series format, which is comprised of brief introductions, lists of required materials and reagents, readily reproducible, step-by-step lab protocols, along with detailed tips on troubleshooting and avoiding known pitfalls, making this book an easy read for investigators at all levels of expertise.

Authoritative and cutting-edge, Microarray Methods for Drug Discovery provides an ideal desk reference for scientists who wish to use microarray technology in order to better understand a variety of biological interactions in their respective fields.

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