Serial Analysis of Gene Expression (SAGE): Methods and Protocols: 387 (Methods in Molecular Biology, 387) - Tapa dura

 
9781588296764: Serial Analysis of Gene Expression (SAGE): Methods and Protocols: 387 (Methods in Molecular Biology, 387)
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ISBN 10: 1588296768 ISBN 13: 9781588296764
Editorial: Humana Press, 2007

Sinopsis

This book facilitates the introduction of SAGE into the laboratory and provides a framework for interpreting and comparing data derived from SAGE experiments. SAGE studies encompass 50,000 tags and can provide detailed knowledge of the 2000 most highly expressed genes in the tissue sample. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world.

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De la contraportada

Serial Analysis of Gene Expression (SAGE): Digital Gene Expression Profiling facilitates the introduction of SAGE into the laboratory, and provides a framework for interpreting and comparing data derived from SAGE experiments. Of the several methods of genetic profiling available, only SAGE measures the expression of both known and unknown genes. SAGE studies encompass 50,000 tags and can provide detailed knowledge of the 2000 most highly expressed genes in the tissue sample. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world. Part 1 is dedicated to experimental procedures of SAGE and related methods including aRNA LongSAGE, SuperSAGE, DeepSAGE, and GMAT. Part 2 provides methods for extraction and filtration of tags, analysis of ditag populations, and completing statistically correct comparisons of gene expression profiles.

Comparative transcriptomics enables scientists to understand the underlying genetics of biological changes such as development, disease, crop yield, and resistance. SAGE analysis is also used to obtain unknown tags, which can be used as gene-specific primers in Rapid Amplification of cDNA Ends (RACE) reactions to generate full-length transcripts for cloning and sequencing. This book will be an indispensable tool for any lab engaged in genetic profiling and comparative transcriptomics, and will help many laboratories to successfully implement tag-based sequencing methods and procedures and obtain comprehensive, useful, and interpretative data.

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Editorial
Humana Press
Año de publicación
2007
Idioma
Inglés
ISBN 10 
1588296768
ISBN 13 
9781588296764
Encuadernación
Tapa dura
Número de páginas
220
Editor
Nielsen Kåre Lehmann
Contacto del fabricante
ProductSafety@springernature.com
ProductSafety@springernature.com

Poststr. 9
Darmstadt
64293
Alemania

Otras ediciones populares con el mismo título

9781597454544: Serial Analysis of Gene Expression (SAGE): Digital Gene Expression Profiling (Methods in Molecular Biology)

Edición Destacada

ISBN 10:  1597454540 ISBN 13:  9781597454544
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