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Microfluidic Techniques: Reviews and Protocols: 321 (Methods in Molecular Biology, 321) - Tapa dura

 
9781588295170: Microfluidic Techniques: Reviews and Protocols: 321 (Methods in Molecular Biology, 321)
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ISBN 10: 1588295176 ISBN 13: 9781588295170
Editorial: Humana Press, 2005

Sinopsis

Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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De la contraportada

Over the last ten years, miniaturization has dramatically shrunk the size of both cellular telephones and chemical instrumentation, and now also holds the promise of developing portable, inexpensive, and disposable analytical systems on a microchip. In Microfluidic Techniques: Reviews and Protocols, hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, polymerase chain reaction (PCR), immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and informative, Microfluidic Techniques: Reviews and Protocols synthesizes the chemistry, biology, and engineering underlying microfluidic devices so that molecular biologists, as well as analytical and clinical chemists, can advance easily from macroscale to microscale biochemical and bioanalytical techniques.

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Editorial
Humana Press
Año de publicación
2005
Idioma
Inglés
ISBN 10 
1588295176
ISBN 13 
9781588295170
Encuadernación
Tapa dura
Número de páginas
260
Editor
Minteer Shelley D.
Contacto del fabricante
Springer Nature Customer Service Center GmbH
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