Pichia Protocols: 389 (Methods in Molecular Biology) - Tapa dura

 
9781588294296: Pichia Protocols: 389 (Methods in Molecular Biology)
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ISBN 10: 1588294293 ISBN 13: 9781588294296
Editorial: Humana Press, 2007

Sinopsis

This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.

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De la contraportada

<p>The use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2<sup>nd</sup> Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent on having both editions in order to utilize the system. The chapters in this indispensable laboratory aid cover a wide range of topics associated with Pichia Pastoris. The use of fermentors to grow Pichia Pastoris and good advice on how to go about the initial processing of material from these cultures is discussed. An extensive discussion on how to formally analyze mutations in essential P. pastoris genes is also presented. The final chapters are for cell biologists who are for cell biologists using the Pichia Pastoris system to investigate protein interactions, or to localize proteins in yeast cells. Pichia Protocols 2<sup>nd</sup> Edition, is an essential tool for researchers working with the Pichia Pastoris system</p>

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Editorial
Humana Press
Año de publicación
2007
Idioma
Inglés
ISBN 10 
1588294293
ISBN 13 
9781588294296
Encuadernación
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Número de edición
2
Número de páginas
284
Editor
Cregg James M.
Contacto del fabricante
no disponible
Persona responsable
no disponible

Otras ediciones populares con el mismo título

9780896034211: Pichia Protocols (Methods in Molecular Biology)

Edición Destacada

ISBN 10:  0896034216 ISBN 13:  9780896034211
Editorial: Humana Press Inc., 1998
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