Baculovirus and Insect Cell Expression Protocols: 1350 (Methods in Molecular Biology, 1350) - Tapa blanda

Libro 66 de 381: Methods in Molecular Biology
 
9781493979783: Baculovirus and Insect Cell Expression Protocols: 1350 (Methods in Molecular Biology, 1350)
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ISBN 10: 1493979787 ISBN 13: 9781493979783
Editorial: Humana, 2018

Sinopsis

The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.

"Sinopsis" puede pertenecer a otra edición de este libro.

De la contraportada

The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.

"Sobre este título" puede pertenecer a otra edición de este libro.

Editorial
Humana
Año de publicación
2018
Idioma
Inglés
ISBN 10 
1493979787
ISBN 13 
9781493979783
Encuadernación
Tapa blanda
Número de páginas
522
Editor
Murhammer David W.
Contacto del fabricante
ProductSafety@springernature.com
ProductSafety@springernature.com

Poststr. 9
Darmstadt
64293
Alemania

Otras ediciones populares con el mismo título

9781493930425: Baculovirus and Insect Cell Expression Protocols: 1350 (Methods in Molecular Biology, 1350)

Edición Destacada

ISBN 10:  1493930427 ISBN 13:  9781493930425
Editorial: Humana, 2016
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