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Part I Construction of Antibody Phage Display Libraries
1. Construction of Human Immune and Naïve scFv Libraries
Jonas Kügler, Florian Tomszak, André Frenzel, and Michael Hust
2. Construction of Naïve and Immune Human Fab Phage Display Library
Noorsharmimi Omar and Theam Soon Lim
3. Construction of Synthetic Antibody Phage Display Libraries
Johan Nilvebrant and Sachdev S. Sidhu
4. Modular Construction of Large Non-Immune Human Antibody Phage Display Libraries from Variable Heavy and Light Chain Gene Cassettes
Nam-Kyung Lee, Scott Bidlingmaier, Yang Su, and Bin Liu
5. Construction of Macaque Immune Libraries
Arnaud Avril, Sebastian Miethe, Michael Hust, and Thibaut Pelat
6. Construction o
f Bovine Immunoglobulin Libraries in the single chain Fragment variable (scFv) FormatUlrike S. Diesterbeck
7. Construction of Rabbit Immune Antibody Libraries
Thi Thu Ha Nguyen, Jong Seo Lee, and Hyunbo Shim
8. Generation of Semi-Synthetic Shark IgNAR Single Domain Antibody Libraries
Julius Grzeschik, Doreen Könning, Simon Krah, Christian Schröter, Martin Empting, Harald Kolmar, and Stefan Zielonka
9. Construction of High-Quality Camel Immune Antibody Libraries
Ema Romão, Vianney Poignavent, Cécile Vincke, Christophe Ritzenthaler, Serge Muyldermans, and Baptiste Monsion
10. Construction of Chicken Antibody Libraries
Jeanni Fehrsen, Susan Wemmer, and Wouter van Wyngaardt
11. Construction and Selection of Affilin Phage Display Libraries
Florian Settele, Madlen Zwarg, Sebastian Fiedler, Daniel Koscheinz, and Eva
Bosse-Doenecke12. Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies
Déborah Caucheteur, Gautier Robin, Vincent Parez, and Pierre Martineau
13. Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display Approach
Zhihao Wu, Brian H. Santich, Hong Liu, Cheng Liu, and Nai-Kong V. Cheung
Part II Selection Strategies for Antibodies
14. Parallelized Antibody Selection in Microtiter Plates
Giulio Russo, Doris Meier, Saskia Helmsing, Esther Wenzel, Fabian Oberle, André Frenzel, and Michael Hust
15. Mass Spectrometry Immuno Assay (MSIA ™) Streptavidin Disposable Automation Research Tips (D.A.R.T's®) Antibody Phage Display Biopanning
Chai Fung Chin, Yee Siew Choong, and Theam Son Lim
16. Magnetic Nanoparticle-Based Semi-Automated Panning for
High-Throughput Antibody SelectionAngela Chiew Wen Ch'ng, Nurul Hamizah binti Hamidon, Zoltán Konthur, and Thean Soon Lim
17. Phage Display and Selections on Cells
Wieland Fahr and André Frenzel
18. Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens
Yang Su, Scott Bidlingmaier, Nam-Kyung Lee, and Bin Liu
19. Antibody Isolation Form a Human Synthetic Combinational and Other Libraries of Single-Chain Antibodies
Almog Bitton, Limor Nahary, and Itai Benhar
20. Screening Phage Display Antibody Libraries using Protein arrays
Ricardo Jara-Acevedo, Paula Díez, María González-González, Rosa María Dégano, Nieves Ibarrola, Rafael Góngora, Alberto Orfao, an
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This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field
of antibody phage display. This volume will also aid in stirring interest and ideas among researchers in this ever-expanding subject."Sobre este título" puede pertenecer a otra edición de este libro.
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field of antibody phage display.This volume will also aid in stirring interest and ideas among researchers in this ever-expanding subject. 576 pp. Englisch. Nº de ref. del artículo: 9781493974467
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Buch. Condición: Neu. Neuware -This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field of antibody phage display.This volume will also aid in stirring interest and ideas among researchers in this ever-expanding subject.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 576 pp. Englisch. Nº de ref. del artículo: 9781493974467
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Buch. Condición: Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field of antibody phage display.This volume will also aid in stirring interest and ideas among researchers in this ever-expanding subject. Nº de ref. del artículo: 9781493974467
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