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Synthetic mRNA: Production, Introduction Into Cells, and Physiological Consequences: 1428 (Methods in Molecular Biology, 1428) - Tapa dura
Sinopsis
This volumepresents detailed laboratory protocols for invitro synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy. Synthetictechniques are described for structural features in mRNA that provide investigationaltools such as fluorescence emission, click chemistry, photo-chemicalcrosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reducedactivation of the innate immune response. Protocols are described for clinicalapplications such as large-scale transfection of dendritic cells, production ofGMP-grade mRNA, redirecting T cell specificity, and use of molecularadjuvants for RNA vaccines. Writtenin the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-steplaboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
SyntheticmRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resourcefor both laboratory investigators and clinicians interested in this powerfuland rapidly evolving technology.
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De la contraportada
This volumepresents detailed laboratory protocols for invitro synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy. Synthetictechniques are described for structural features in mRNA that provide investigationaltools such as fluorescence emission, click chemistry, photo-chemicalcrosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reducedactivation of the innate immune response. Protocols are described for clinicalapplications such as large-scale transfection of dendritic cells, production ofGMP-grade mRNA, redirecting T cell specificity, and use of molecularadjuvants for RNA vaccines. Writtenin the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-steplaboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
SyntheticmRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resourcefor both laboratory investigators and clinicians interested in this powerfuland rapidly evolving technology.
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Synthetic mRNA (eng)
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Synthetic mRNA
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Condición: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the expertsIncludes cutting-edge methods and protocolsProvides ste. Nº de ref. del artículo: 107269846
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Synthetic mRNA
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Springer New York Mai 2016, 2016
ISBN 10: 1493936239
ISBN 13: 9781493936236
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Librería: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Alemania
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volumepresents detailed laboratory protocols for invitro synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy. Synthetictechniques are described for structural features in mRNA that provide investigationaltools such as fluorescence emission, click chemistry, photo-chemicalcrosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reducedactivation of the innate immune response. Protocols are described for clinicalapplications such as large-scale transfection of dendritic cells, production ofGMP-grade mRNA, redirecting T cell specificity, and use of molecularadjuvants for RNA vaccines. Writtenin the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessarymaterials and reagents, step-by-steplaboratory protocols, and tips on troubleshooting and avoiding known pitfalls.SyntheticmRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resourcefor both laboratory investigators and clinicians interested in this powerfuland rapidly evolving technology. 332 pp. Englisch. Nº de ref. del artículo: 9781493936236
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Synthetic mRNA: Production, Introduction Into Cells, and Physiological Consequences (Methods in Molecular Biology, 1428)
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Condición: New. In English. Nº de ref. del artículo: ria9781493936236_new
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Synthetic mRNA : Production, Introduction Into Cells, and Physiological Consequences
Impresión bajo demandaLibrería: AHA-BUCH GmbH, Einbeck, Alemania
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Buch. Condición: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - This volumepresents detailed laboratory protocols for invitro synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy. Synthetictechniques are described for structural features in mRNA that provide investigationaltools such as fluorescence emission, click chemistry, photo-chemicalcrosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reducedactivation of the innate immune response. Protocols are described for clinicalapplications such as large-scale transfection of dendritic cells, production ofGMP-grade mRNA, redirecting T cell specificity, and use of molecularadjuvants for RNA vaccines. Writtenin the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessarymaterials and reagents, step-by-steplaboratory protocols, and tips on troubleshooting and avoiding known pitfalls.SyntheticmRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resourcefor both laboratory investigators and clinicians interested in this powerfuland rapidly evolving technology. Nº de ref. del artículo: 9781493936236
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Synthetic mRNA
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Condición: New. Editor(s): Rhoads, Robert E. Series: Methods in Molecular Biology. Num Pages: 332 pages, 23 black & white illustrations, 39 colour illustrations, 5 black & white tables, 37 colou. BIC Classification: MFN; PSAK1; PSD. Category: (G) General (US: Trade). Dimension: 254 x 178 x 19. Weight in Grams: 819. . 2016. Hardback. . . . . Nº de ref. del artículo: V9781493936236
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Synthetic mRNA
Impresión bajo demandaLibrería: buchversandmimpf2000, Emtmannsberg, BAYE, Alemania
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Buch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volumepresents detailed laboratory protocols for invitro synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy. Synthetictechniques are described for structural features in mRNA that provide investigationaltools such as fluorescence emission, click chemistry, photo-chemicalcrosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reducedactivation of the innate immune response. Protocols are described for clinicalapplications such as large-scale transfection of dendritic cells, production ofGMP-grade mRNA, redirecting T cell specificity, and use of molecularadjuvants for RNA vaccines. Writtenin the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessarymaterials and reagents, step-by-steplaboratory protocols, and tips on troubleshooting and avoiding known pitfalls.SyntheticmRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resourcefor both laboratory investigators and clinicians interested in this powerfuland rapidly evolving technology.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 332 pp. Englisch. Nº de ref. del artículo: 9781493936236
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Synthetic mRNA: Production, Introduction Into Cells, and Physiological Consequences (Methods in Molecular Biology)
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Condición: New. pp. 320. Nº de ref. del artículo: 26375148857
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Synthetic mRNA: Production, Introduction Into Cells, and Physiological Consequences (Methods in Molecular Biology)
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Condición: New. Print on Demand pp. 320. Nº de ref. del artículo: 371977958
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Synthetic mRNA: Production, Introduction Into Cells, and Physiological Consequences (Methods in Molecular Biology)
Impresión bajo demandaLibrería: Biblios, Frankfurt am main, HESSE, Alemania
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Condición: New. PRINT ON DEMAND pp. 320. Nº de ref. del artículo: 18375148851
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