Cell Analysis: Volume 1 - Tapa blanda

Sinopsis

1 Quantification of Red Blood Cell Morphology.- I. History.- II. Details of Red Cell Measurements.- III. Cell Sample Population Distributions.- IV. Discussion and Summary.- References.- 2 Laser Microirradiation and Computer Video Optical Microscopy in Cell Analysis.- I. Introduction.- II. Laser Microbeams.- III. Computer-Enhanced Video Microscopy for Laser Microsurgery.- A. Contrast Enhancement.- B. Edge Enhancement.- C. Pseudocolor Enhancement.- IV. Chromosome Microsurgery.- A. Mitotic Organelles.- B. Cytoplasm.- V. Plant Cell Development (Chloroplast Irradiation).- VI. Developmental Cellular Neurobiology.- VII. Pattern Formation.- VIII. Conclusions.- References.- 3 Combination of Two Physical Parameters for the Identification and Separation of Lymphocyte Subsets.- I. Introduction.- II. Methods.- A. Preparation of Cell Suspensions.- B. Fractionation of Cell Suspensions by Free-Flow Preparative Electrophoresis.- C. Separation of Cells by Isopycnic Centrifugation in a Percoll Gradient.- D. Cell Enumeration and Size Distribution Analysis.- E. Construction of the Fingerprints.- F. Interpretation of the Fingerprints.- III. Applications.- A. Fingerprint Analysis of Murine Thymocytes.- B. Fingerprint Analysis of Murine Splenocytes.- C. Fingerprint Analysis of Murine Lymph Node Cells.- IV. Conclusion.- References.- 4 Electrical Sizing of Cells in Suspension.- I. Introduction.- II. Theory.- A. Electric Field.- B. Hydrodynamic Field.- C. Cell Volume.- D. Shape and Orientation.- E. Membrane Conductance.- F. Electrical Size.- III. Instrumentation.- A. The Transducer.- B. The Amplifiers.- C. The Selector.- D. Data Handling.- E. Auxiliary Units.- IV. Operation.- A. Cell Suspensions.- B. Orifice Dimensions.- C. Amplification.- D. Calibration.- V. Applications.- A. Fast Kinetics.- B. High Fields.- C. Membrane Permeability.- VI. The Future.- A. Sensitivity.- B. Multiparameter Systems.- References.- 5 Light Scattering Analysis of Single Cells.- I. Introduction.- II. Theoretical Considerations.- A. Scattering Theory.- B. Coated Sphere Model Tests.- C. Expected Detector Responses.- III. Recent Applications of Forward and 90° Scatter.- A. Human Peripheral Blood.- B. Osmolarity Effects.- C. Hematopoietic Stem Cells.- D. T-Lymphocyte Subclasses.- E. Non-Hodgkin's Lymphomas.- F. Cervical Cell Analysis with 90° Scatter.- G. Sputum Samples.- IV. Multiangle and Multiwavelength Scattering.- A. Multiangle Scattering.- B. Two-Color Light Scattering.- V. Fourier Transform Techniques.- VI. Polarization Studies-New Directions.- A. Polarization Transformations.- B. Circular Intensity Differential Scattering.- VII. Conclusion.- References.- 6 Methods for Measuring Leukocyte Locomotion.- I. Introduction.- II. Visual Assays.- A. Time-Lapse Filming.- B. Indirect (Non-Time-Lapse) Methods.- C. Analysis of Cell Movement.- D. The Orientation Assay.- E. The Candida Assay.- III. The Micropore Filter Assay.- A. Principle.- B. Apparatus.- C. Measurement of Leukocyte Locomotion in Filter Assays.- D. The Checkerboard Assay.- E. Automated Method.- IV. The Under-Agarose Assay.- V. Miscellaneous Techniques.- A. The Capillary-Tube Assay for Macrophage Locomotion.- B. The Agarose Microdroplet Assay.- VI. Factors Influencing Locomotion.- A. Cell-Substratum Interactions.- B. Composition of the Fluid Phase.- C. Cell-Cell Interactions.- References.- 7 Sizing of Cells by the Electrical Resistance Pulse Technique: Methodology and Application in Cytometric Systems.- I. Introduction.- A. Reasons for Measuring Size of Biological Cells.- B. A Short Review on the History of Electrical Sizing.- II. Basic Events and Problems in Electrically Sizing Transducers.- A. The Basic Coulter Effect.- B. Fundamental Relations of Particle Volume, Electrical Resistivity, Resistance Change, and Pulse Height.- C. Flow Conditions and Flow Line Coordination in the Sensitive Zone.- D. Behavior of Biological Particles in the Sizing Transducer.- III. Instrumentation.- A. General Conditions and Review.- B. T

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