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Sinopsis
This detailed volume provides protocols for the identification, characterization, and application of degrons, central to controlling protein abundance and cellular homeostasis, in a range of organisms from bacteria to humans. Beginning with reporter assays and tools used in human cells to study the involvement of the ubiquitin-proteasome system or ubiquitin-independent degradation pathways, the book continues by exploring methods for chemical and enzymatic synthesis, characterization of degrons and their mimics, methods for other selected eukaryotes, including plants and employing inducible degradation in nematodes and trypanosomes, as well as methods used for studying N- and C-degrons in bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Degrons: Methods and Protocols serves as an ideal guide for researchers working on protein degradation or using degrons to study or control protein function.
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De la contraportada
This detailed volume provides protocols for the identification, characterization, and application of degrons, central to controlling protein abundance and cellular homeostasis, in a range of organisms from bacteria to humans. Beginning with reporter assays and tools used in human cells to study the involvement of the ubiquitin-proteasome system or ubiquitin-independent degradation pathways, the book continues by exploring methods for chemical and enzymatic synthesis, characterization of degrons and their mimics, methods for other selected eukaryotes, including plants and employing inducible degradation in nematodes and trypanosomes, as well as methods used for studying N- and C-degrons in bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Degrons: Methods and Protocols serves as an ideal guide for researchers working on protein degradation or using degrons to study or control protein function.
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Degrons
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Degrons
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume provides protocols for the identification, characterization, and application of degrons, central to controlling protein abundance and cellular homeostasis, in a range of organisms from bacteria to humans. Beginning with reporter assays and tools used in human cells to study the involvement of the ubiquitin-proteasome system or ubiquitin-independent degradation pathways, the book continues by exploring methods for chemical and enzymatic synthesis, characterization of degrons and their mimics, methods for other selected eukaryotes, including plants and employing inducible degradation in nematodes and trypanosomes, as well as methods used for studying N- and C-degrons in bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.Authoritative and practical, Degrons: Methods and Protocols serves as an ideal guide for researchers working on protein degradation or using degrons to study or control protein function. 272 pp. Englisch. Nº de ref. del artículo: 9781071655078
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Degrons (Hardcover)
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Hardcover. Condición: new. Hardcover. This detailed volume provides protocols for the identification, characterization, and application of degrons, central to controlling protein abundance and cellular homeostasis, in a range of organisms from bacteria to humans. Beginning with reporter assays and tools used in human cells to study the involvement of the ubiquitin-proteasome system or ubiquitin-independent degradation pathways, the book continues by exploring methods for chemical and enzymatic synthesis, characterization of degrons and their mimics, methods for other selected eukaryotes, including plants and employing inducible degradation in nematodes and trypanosomes, as well as methods used for studying N- and C-degrons in bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.Authoritative and practical, Degrons: Methods and Protocols serves as an ideal guide for researchers working on protein degradation or using degrons to study or control protein function. This item is printed on demand. Shipping may be from multiple locations in the US or from the UK, depending on stock availability. Nº de ref. del artículo: 9781071655078
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Hardcover. Condición: new. Hardcover. This detailed volume provides protocols for the identification, characterization, and application of degrons, central to controlling protein abundance and cellular homeostasis, in a range of organisms from bacteria to humans. Beginning with reporter assays and tools used in human cells to study the involvement of the ubiquitin-proteasome system or ubiquitin-independent degradation pathways, the book continues by exploring methods for chemical and enzymatic synthesis, characterization of degrons and their mimics, methods for other selected eukaryotes, including plants and employing inducible degradation in nematodes and trypanosomes, as well as methods used for studying N- and C-degrons in bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.Authoritative and practical, Degrons: Methods and Protocols serves as an ideal guide for researchers working on protein degradation or using degrons to study or control protein function. This item is printed on demand. Shipping may be from our Sydney, NSW warehouse or from our UK or US warehouse, depending on stock availability. Nº de ref. del artículo: 9781071655078
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Degrons: Methods and Protocols (Methods in Molecular Biology, 3069)
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Degrons: Methods and Protocols (Volume 3069)
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Degrons : Methods and Protocols
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Buch. Condición: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - This detailed volume provides protocols for the identification, characterization, and application of degrons, central to controlling protein abundance and cellular homeostasis, in a range of organisms from bacteria to humans. Beginning with reporter assays and tools used in human cells to study the involvement of the ubiquitin-proteasome system or ubiquitin-independent degradation pathways, the book continues by exploring methods for chemical and enzymatic synthesis, characterization of degrons and their mimics, methods for other selected eukaryotes, including plants and employing inducible degradation in nematodes and trypanosomes, as well as methods used for studying N- and C-degrons in bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.Authoritative and practical, Degrons: Methods and Protocols serves as an ideal guide for researchers working on protein degradation or using degrons to study or control protein function. Nº de ref. del artículo: 9781071655078
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Buch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This detailed volume provides protocols for the identification, characterization, and application of degrons, central to controlling protein abundance and cellular homeostasis, in a range of organisms from bacteria to humans. Beginning with reporter assays and tools used in human cells to study the involvement of the ubiquitin-proteasome system or ubiquitin-independent degradation pathways, the book continues by exploring methods for chemical and enzymatic synthesis, characterization of degrons and their mimics, methods for other selected eukaryotes, including plants and employing inducible degradation in nematodes and trypanosomes, as well as methods used for studying N- and C-degrons in bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.Authoritative and practical, Degrons: Methods and Protocols serves as an ideal guide for researchers working on protein degradation or using degrons to study or control protein function.Springer Nature Customer Service Center GmbH, Europaplatz 3, 69115 Heidelberg 272 pp. Englisch. Nº de ref. del artículo: 9781071655078
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Hardcover. Condición: new. Hardcover. This detailed volume provides protocols for the identification, characterization, and application of degrons, central to controlling protein abundance and cellular homeostasis, in a range of organisms from bacteria to humans. Beginning with reporter assays and tools used in human cells to study the involvement of the ubiquitin-proteasome system or ubiquitin-independent degradation pathways, the book continues by exploring methods for chemical and enzymatic synthesis, characterization of degrons and their mimics, methods for other selected eukaryotes, including plants and employing inducible degradation in nematodes and trypanosomes, as well as methods used for studying N- and C-degrons in bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.Authoritative and practical, Degrons: Methods and Protocols serves as an ideal guide for researchers working on protein degradation or using degrons to study or control protein function. This item is printed on demand. Shipping may be from our UK warehouse or from our Australian or US warehouses, depending on stock availability. Nº de ref. del artículo: 9781071655078
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Degrons: Methods and Protocols: 3069 (Methods in Molecular Biology, 3069)
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