Bacterial Virulence: Methods and Protocols: 2427 (Methods in Molecular Biology) - Tapa dura

 
9781071619704: Bacterial Virulence: Methods and Protocols: 2427 (Methods in Molecular Biology)
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ISBN 10: 1071619705 ISBN 13: 9781071619704
Editorial: Humana, 2022

Sinopsis

Part I: Molecular Biology and Bioinformatics Methods

 

1. Generation of Markerless Gene Deletion Mutants in Listeria monocytogenes Using a Mutated PheS for Counterselection

            Shai Ran Sapir, Etai Boichis, and Anat A. Herskovits

 

2. A Rapid Fluorescence-Based Screen to Identify Regulators and Components of Interbacterial Competition Mechanisms in Bacteria

            Daniel Tchelet and Dor Salomon

 

3. Predicting Type III Effector Proteins Using the Effectidor Web-Server

            Naama Wagner, Doron Teper, and Tal Pupko

 

4. Assay for Type III Secretion in Escherichia coli

            Bosko Mitrovic and Neta Sal-Man

 

5. Profiling of Secreted Type 3 Secretion System Substrates by Salmonella enterica

            Rivka Shem-Tov and Ohad Gal-Mor

 

Part II: Cell Culture and Organoid Models of Infection

 

6. Analysis of SPI-1 Dependent Type III Secretion and Injection Using a NanoLuc Luciferase-Based Assay

            Sara Vilela Pais, Sibel Westerhausen, Erwin Bohn, and Samuel Wagner

 

7. Mycobacterium tuberculosis Infection of THP-1 Cells: A Model for High Content Analysis of Intracellular Growth and Drug Susceptibility

            Leah Rankine-Wilson, Céline Rens, Henok Asfaw Sahile, and Yossef Av-Gay

 

8. Bone Marrow-Derived Macrophage (BMDM) Infection by Listeria monocytogenes

            Etai Boichis, Shai Ran Sapir, and Anat A. Herskovits

 

9. Preparation and Inflammasome Activation of Murine Bone Marrow-Derived and Resident Peritoneal Macrophages

            Izumi Sasaki and Tsuneyasu Kaisho

 

10. Flow Cytometry-Based Single Cell Analyses of Bacterial Adaption to Intracellular Environments

            Marc Schulte and Michael Hensel

 

11. Quantification of Microbial Fluorescent Sensors during Live Intracellular Infections

            Erez Mills and Erik Petersen

 

12. Dissecting Human Blood Immune Cells Response to Intracellular Infection Using Single Cell RNA-Sequencing

            Shelly Hen-Avivi and Roi Avraham

 

13. Salmonella enterica Infection of Human and Mouse Colon Organoid-Derived Monolayers

            Erin C. Boyle, Eva J. Wunschel, and Guntram A. Grassl

 

Part III: In Vivo Models of Infection

 

14. Analysis of Salmonella enterica Adhesion to Leaves of Corn Salad or Lettuce

            Laura Elpers and Michael Hensel

 

15. Methods for Using the Galleria mellonella Invertebrate Model to Probe Enterococcus faecalis Pathogenicity

            Ling Ning Lam, Debra N. Brunson, Jessica K. Kajfasz, an

"Sinopsis" puede pertenecer a otra edición de este libro.

De la contraportada

This detailed volume explores the field of bacterial virulence and the effort to understand how microbial interaction with a host results in the pathology of a specific disease. This collection of selected protocols includes advanced molecular biology and bioinformatics methods, cell culture and organoid models of infection, as well as in vivo infection models that are useful to study the interaction of pathogens with plants, insects, avian, and mammalian hosts. Written for the highly successful <i>Methods in Molecular Biology </i>series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.&nbsp;<div><br></div><div>Authoritative and beneficial, <i>Bacterial Virulence: Methods and Protocols</i> serves as an ideal guide for researchers seeking to promote and further develop the exciting and continuously evolving field of bacterial virulence.</div><div><br></div><div>Chapter 19 is&nbsp;available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.<br></div>

"Sobre este título" puede pertenecer a otra edición de este libro.

Editorial
Humana
Año de publicación
2022
Idioma
Inglés
ISBN 10 
1071619705
ISBN 13 
9781071619704
Encuadernación
Tapa dura
Número de páginas
280
Editor
Gal-Mor Ohad
Contacto del fabricante
Springer Nature Customer Service Center GmbH
ProductSafety@springernature.com

Europaplatz 3,69115 Heidelberg, Germany
Heidelberg
69115
Alemania

Otras ediciones populares con el mismo título

9781071619735: Bacterial Virulence: Methods and Protocols: 2427 (Methods in Molecular Biology)

Edición Destacada

ISBN 10:  107161973X ISBN 13:  9781071619735
Editorial: Springer-Verlag New York Inc., 2023
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