Structure and Function of Membrane Proteins: 2302 (Methods in Molecular Biology) - Tapa blanda

Sinopsis

1. Expression and Purification of Human Mitochondrial Intramembrane Protease PARL

            Elena Arutyunova, Laine Lysyk, Melissa Morrison, Cory Brooks, and M. Joanne Lemieux

2. Reconstitution of Detergent-Solubilized Membrane Proteins into Proteoliposomes and Nanodiscs for Functional and Structural Studies

            Kerry M. Strickland, Kasahun Neselu, Arshay J. Grant, Carolann L. Espy, Nael A. McCarty, and Ingeborg Schmidt-Krey

3. Biochemical Characterization of GPCR-G Protein Complex Formation

            Filip Pamula and Ching-Ju Tsai

4. Electrophysiological Approaches for the Study of Ion Channel Function

            Guiying Cui, Kirsten A. Cottrill, and Nael A. McCarty

5. Isothermal Titration Calorimetry of Membrane Proteins

            Han N. Vu, Alan J. Situ, and Tobias S. Ulmer

6. Atomic Force Microscopy Reveals Membrane Protein Activity at the Single Molecule Level

            Kanokporn Chattrakun, Katherine G. Schaefer, Lucas S. Chandler, Brendan P. Marsh, and Gavin M. King

7. Structure Determination of Membrane Proteins Using X-Ray Crystallography

            Evan Billings, Karl Lundquist, Claire Overly, Karthik Srinivasan, and Nicholas Noinaj

8. Studying Membrane Protein Structures by MicroED

            Michael W. Martynowycz and Tamir Gonen

9. Single-Particle Cryo-EM of Membrane Proteins

            Dovile Januliene and Arne Moeller

10. Helical Membrane Protein Crystallization in the New Era of Electron Cryo-Microscopy

            Mary D. Hernando, Joseph O. Primeau, and Howard S. Young

11. NMR Spectroscopic Studies of Ion Channels in Lipid Bilayers: Sample Preparation Strategies Exemplified by the Voltage Dependent Anion Channel

            Robert Silvers and Matthew Eddy

12. Preparation of a Deuterated Membrane Protein for Small-Angle Neutron Scattering

            Yuqi Wu, Kevin L. Weiss, and Raquel L. Lieberman

13. Preparing Membrane Proteins for Simulation Using CHARMM-GUI

            Yupeng Li, Jinchan Liu, and James C. Gumbart

14. Coarse-Grained Molecular Dynamics Simulations of Membrane Proteins: A Practical Guide

            William Glass, Jonathan W. Essex, Franca Fraternali, James Gebbie-Rayet, Irene Marzuoli, Marley L. Samways, Philip C. Biggin, and Syma Khalid

15. Continuous Constant pH Molecular Dynamics Simulations of Transmembrane Proteins

            Yandong Huang, Jack A. Henderson, and Jana Shen

16. Molecular Dynamics-Based Thermodynamic and Kinetic Characterization of Membrane Protein Conformational Transitions

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