Ewing Sarcoma: Methods and Protocols: 2226 (Methods in Molecular Biology) - Tapa blanda

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<p>This volume aims at providing a selection of the most effective and widely employed protocols used in the Ewing sarcoma field. Chapters are divided into five sections detailing the&nbsp;molecular biology techniques used to analyze Ewing sarcoma including tissue preservation and methods used in morpho-molecular diagnostics and routine immune-histological analysis. Additional wet-lab protocols comprise i<i>n vitro&nbsp;</i>techniques&nbsp;including re-expression and functional analyses of &nbsp;target genes together with&nbsp;<i>in vivo</i>&nbsp;tools and models such as patient-derived xenografts (PDX) and orthotopic xenografts for metastasis assessment in Ewing sarcoma.&nbsp;A full section on bioinformatic approaches to Ewing sarcoma covering ChIPseq, epigenetic and systems biology analyses finalizes this volume.&nbsp;Written in the highly successful&nbsp;<i>Methods in Molecular Biology&nbsp;</i>series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.</p><p>&nbsp;</p>Cutting-edge and clearly written,&nbsp;<i>Ewing sarcoma: Methods and Protocols</i>&nbsp;aims to provide methods to further the study of&nbsp;Ewing sarcoma and bring researchers one step forward in the quest to fight this devastating disease.<p></p>