Expert researchers from academia, industry, and leading genome centers provide a comprehensive generic set of protocols for gene hunting and genome analysis. Drawing on emerging technologies in the fields of bioinformatics and proteomics, these protocols cover not only those traditionally recognized as genomics, but also early therapeutic approaches exploiting the potential of gene therapy. The highlights include methods for the analysis of differential gene expression, SNP detection, comparative genomic hybridization, and the functional analysis of genes, as well as the use of bioinformatics for gene identification and the prediction of protein function.
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The two editors of this volume have invited contributors from a variety of authors to write a laboratory manual dealing exclusively with the application of genomic methods in eukaryotes. Each chapter is presented in a standardized fashion, beginning with an introduction followed by a list of all the materials needed (including the machines and software) a detailed experimental protocol, notes (troubleshooting), and a list of references. it will be of value for those requiring step-by-step cookbook procedures for some of the methodologies described, and this definitive and authorative volume belongs in the library of every quality research institution where genomics is performed. - CHEMBIOCHEM
Expert researchers from academia, industry, and leading genome centers provide a comprehensive generic set of protocols for gene hunting and genome analysis. Drawing on emerging technologies in the fields of bioinformatics and proteomics, these protocols cover not only those traditionally recognized as genomics, but also early therapeutic approaches exploiting the potential of gene therapy. Highlights include methods for the analysis of differential gene expression, SNP detection, comparative genomic hybridization, and the functional analysis of genes, as well as the use of bioinformatics for gene identification and the prediction of protein function.
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