Transgenesis Techniques: Principles and Protocols: v. 180 (Methods in Molecular Biology) - Tapa dura

 
9780896036963: Transgenesis Techniques: Principles and Protocols: v. 180 (Methods in Molecular Biology)
Tapa dura
ISBN 10: 0896036960 ISBN 13: 9780896036963
Editorial: Humana Press Inc., 2002

Sinopsis

This revised and updated edition of a much-acclaimed classic presents a collection of readily reproducible techniques for the generation and analysis of transgenic animals, with emphasis on the manipulation of the mammalian genome. It concentrates on the creation and maintenance of genetically modified murine strains, providing access to the germline by both conventional pronuclear injection, and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms.

"Sinopsis" puede pertenecer a otra edición de este libro.

Críticas

From Reviews of the First Edition a must in any laboratory - Cellular and Molecular Biology should be useful to anyone currently involved in using transgenesis as a technique - Journal of Heredity Second Edition: ...the book remains an impressive and interesting book that will be a useful reference to molecular and developmental geneticists at all levels and potential tool for teaching graduate students and advanced undergraduates. - Human Genetics It will provide a valuable source of information. It will also remind you of the enormous potential that transgenesis has to offer the biologist. Transgenic research will underpin many future applications from evaluating viral vectors for gene therapy and vaccination to bioreactors for the production of proteins, from models of disease to screening tools for biological activity, from genome mapping to gene function. This book details protocols to let you participate in this. Cloning and Stem Cells

Reseña del editor

This revised and updated edition of a much-acclaimed classic presents a collection of readily reproducible techniques for the generation and analysis of transgenic animals, with emphasis on the manipulation of the mammalian genome. It concentrates on the creation and maintenance of genetically modified murine strains, providing access to the germline by both conventional pronuclear injection, and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms.

"Sobre este título" puede pertenecer a otra edición de este libro.

Editorial
Humana Press Inc.
Año de publicación
2002
Idioma
Inglés
ISBN 10 
0896036960
ISBN 13 
9780896036963
Encuadernación
Tapa dura
Número de páginas
368
Editor
Clarke Alan
Contacto del fabricante
no disponible
Persona responsable
no disponible