Artículos relacionados a Pichia Protocols (Methods in Molecular Biology)

Pichia Protocols (Methods in Molecular Biology) - Tapa dura

Libro 44 de 381: Methods in Molecular Biology

Higgins, C

 
9780896034211: Pichia Protocols (Methods in Molecular Biology)
Tapa dura
ISBN 10: 0896034216 ISBN 13: 9780896034211
Editorial: Humana Press Inc., 1998

Sinopsis

David Higgins and James Cregg compile for the first time an all-inclusive collection of key experimental procedures to manipulate Pichia pastoris for gene expression purposes. The methods - developed by world-class researchers in their own laboratories - contain detailed descriptions of available expression strains, as well as vectors and step-by-step instructions for the construction of expression strains and for their use in the production of foreign proteins from shake-flask or fermentor cultures. General procedures for the purification of foreign proteins are also provided, along with protocols for analyzing foreign protein products for glycosylation and subcellular location. Its detailed instructions will permit any researcher in molecular biology to quickly construct an expression strain that will produce a desired foreign protein, and to use this state-of-the-art molecular and cellular biological system successfully in every area of biological research today.

"Sinopsis" puede pertenecer a otra edición de este libro.

Críticas

In this valuable Methods in Molecular Biology volume, the editors have assembled an international cast of Pichia experts who provide detailed protocols, advice, and theory on recombinant expression in this methylotrophic yeast...a focused writing style that is enjoyable to read. Molecular geneticists are afforded the essential information to achieve success in vector construction, transformation and screening. - The Quarterly Review of Biology

Reseña del editor

David Higgins and James Cregg compile for the first time an all-inclusive collection of key experimental procedures to manipulate Pichia pastoris for gene expression purposes. The methods-developed by world-class researchers in their own laboratories-contain detailed descriptions of available expression strains, as well as vectors and step-by-step instructions for the construction of expression strains and for their use in the production of foreign proteins from shake-flask or fermentor cultures. General procedures for the purification of foreign proteins are also provided, along with protocols for analyzing foreign protein products for glycosylation and subcellular location. Its detailed instructions will permit any researcher in molecular biology to quickly construct an expression strain that will produce a desired foreign protein, and to use this state-of-the-art molecular and cellular biological system successfully in every area of biological research today.

"Sobre este título" puede pertenecer a otra edición de este libro.

Editorial
Humana Press Inc.
Año de publicación
1998
Idioma
Inglés
ISBN 10 
0896034216
ISBN 13 
9780896034211
Encuadernación
Tapa dura
Número de páginas
284
Contacto del fabricante
no disponible
Persona responsable
no disponible