Ribozyme Protocols: 74 (Methods in Molecular Biology, 74) - Tapa dura

Turner, P. C.

 
9780896033894: Ribozyme Protocols: 74 (Methods in Molecular Biology, 74)
Tapa dura
ISBN 10: 0896033899 ISBN 13: 9780896033894
Editorial: Humana Press, 1997

Sinopsis

The purpose of Ribozyme Protocols is to provide a helpful compilation of protocols that will be of use-^not only to those with some experience of ribozymes-^but also to those wishing to use ribozymes for the first time. Although it is usually impossible to cover every aspect of a scientific field, I believe this book approaches that ideal and should help all readers perform meaningful experiments using ribozymes. To design ribozymes, one must consider whether the target site will be accessible; this task can be facilitated by using computer programs that pre­ dict the folding of the target RNA. Such programs are detailed in Chapters 2 and 3. If the chosen target is an RNA virus that can mutate rapidly, it makes sense to consider those parts of the genome that are least likely to change during viral replication. An example of how this can be done is described in Chapter 4. Although computer analysis may be a useful starting point to select tar­ get sites, there seems, at the moment, to be no guarantee that any particular chosen site will be efficiently cleaved. Some workers have deliberately bypassed this problem by using libraries of ribozyme sequences and by select­ ing those that actually hybridize to and/or cleave the target; these methods are described in Chapters 5 and 6.

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De la contraportada

Ribozyme Protocols brings together a broad spectrum of essential and easily reproducible techniques currently used by leading researchers to manipulate RNA molecules and to inactivate gene expression in vivo. The methods collected here range from theoretical design to clinical application and include the synthesis and assay of ribozymes, as well as their use both in vitro and in vivo. Each method is presented by an expert with substantial hands-on experience and in far greater detail than is generally to be found in the research literature.

Ribozyme Protocols will help all investigators perform meaningful and successful experiments using ribozymes in both clinical and basic research, as well as launch fresh application initiatives. It is certain to be found indispensable not only by those workers new to the field, but also by experienced researchers who wish to expand their range of expertise.

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Editorial
Humana Press
Año de publicación
1997
Idioma
Inglés
ISBN 10 
0896033899
ISBN 13 
9780896033894
Encuadernación
Tapa dura
Número de páginas
512
Editor
Turner Philip C.
Contacto del fabricante
no disponible
Persona responsable
no disponible

Otras ediciones populares con el mismo título

9781489942852: Ribozyme Protocols: 74 (Methods in Molecular Biology)

Edición Destacada

ISBN 10:  1489942858 ISBN 13:  9781489942852
Editorial: Humana, 2013
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