Protocols for Nucleic Acid Analysis by Nonradioactive Probes: 28 (Methods in Molecular Biology) - Tapa blanda

Isaac

 
9780896032545: Protocols for Nucleic Acid Analysis by Nonradioactive Probes: 28 (Methods in Molecular Biology)
Tapa blanda
ISBN 10: 089603254X ISBN 13: 9780896032545
Editorial: Humana Press Inc., 1993

Sinopsis

This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of probe systems (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection). Other techniques treated in detail include hybridization in situ to chromosomes and transcribed RNA, newer and less conventional technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse dot blots, bacterial identification using magnetic bead and dipstick assays, and nucleic acid sequence-based amplification.

"Sinopsis" puede pertenecer a otra edición de este libro.

Críticas

...this book provides a collection of good protocols which are in general clearly written. They can be easily employed on the bench without being a specialist in a particular fields. - FEBS Letters

Reseña del editor

This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of probe systems (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection). Other techniques treated in detail include hybridization in situ to chromosomes and transcribed RNA, newer and less conventional technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse dot blots, bacterial identification using magnetic bead and dipstick assays, and nucleic acid sequence-based amplification.

"Sobre este título" puede pertenecer a otra edición de este libro.

Editorial
Humana Press Inc.
Año de publicación
1993
Idioma
Inglés
ISBN 10 
089603254X
ISBN 13 
9780896032545
Encuadernación
Tapa blanda
Número de páginas
280
Contacto del fabricante
no disponible
Persona responsable
HUMANA SPAIN S.L.
https://www.humana-spain.org/?lang=en

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Madrid
28016
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