Completion of sequencing of the human genome, as well as those of many other organisms, has opened the door for exploration of the proteome, the many thousands of interacting proteins in a given organism. Critical to tackling the complexity of the proteome is a workable strategy using reliable and tested protocols for identifying, isolating, and quantifying proteins in cells and cell pathways and for performing functional assays. This book provides an authoritative and wide-ranging protocol-based approach to proteomics. Presented as a logical strategy for analyzing proteomes, the book provides information about protein structure and numerous methods for the preparation and analysis of protein samples ranging from electrophoresis and mass spectrometry to protein chips and informatics. Extensive background information and references are provided regarding the theoretic aspects of the techniques presented as well as their applications. This manual aims to offer a useful laboratory tool for genetics and molecular biology investigators moving from studies of genomics and genotype to those of proteomics and phenotype. It should also be valuable for cell biologists, developmental biologists and neuroscientists exploring the intricacies of the proteome and a useful resource for protein chemists and biochemists.
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