A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. A bestseller in its first edition, this second edition provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution.
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A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. A bestseller in its first edition, this second edition provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution.
The polymerase chain reaction (or PCR) is a technique used by scientists to amplify minute amounts of DNA. This procedure has a wide range of applications. As well as being a tool in the research laboratory, PCR can be used to amplify DNA from a single human hair for use in genetic fingerprinting, or to study the DNA from the remains of extinct animals. PCR also has applications in diagnosing disease, detecting contamination of food, and in monitoring pollution in the environment. This book provides the reader with detailed descriptions of the most recent and innovative protocols and applications for this technology.
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