Cell Engineering: Transient Expression: 2 - Tapa dura

Libro 7 de 10: Cell Engineering
 
9780792365969: Cell Engineering: Transient Expression: 2
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ISBN 10: 0792365968 ISBN 13: 9780792365969
Editorial: Kluwer Academic Publishers, 2000

Sinopsis

This volume focuses on the transient gene expression technologies for recombinant protein production and gene therapy. Topics covered range from the use of baculovirus, adenovirus, adeno-associated virus and alphavirus vectors in biopharmaceutical, genomics and gene delivery research, to large-scale transfection of plasmid DNA into cultured cells for bioreactor applications. The book illuminates all the scientific and technological issues involved in the research, development, production, and human use of viral and non-viral vectors for in vitro and in vivo expression of recombinant proteins, thus making it an useful reference for cell culture technologists, virologists, and scientists interested in gene therapy.

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Reseña del editor

The advantages of the baculovirus system are rooted in the properties of the virus and the host (insect, or cell lines derived from it). During the normal infection cycle, two forms of the virus are produced: an early budded virus (BY) form (Kost et al. , 2000), in which the viral DNA and structural proteins are surrounded by membrane derived from the infected cell; and a late occluded form (occlusion-derived virus, ODy), consisting of enveloped viral cores which are embedded in a crystal matrix of viral proteins. The principal component of the matrix is the abundantly expressed protein polyhedrin. The budded virus rapidly spreads the infection from cell to cell within the insect host, resulting ultimately in the complete liquefaction of the host, and release of occluded virus into the environment. The occluded form protects the released virus, allowing it to survive for long periods in the environment until ingested by another host. In the alkaline environment ofthe insect gut, the protective protein matrix is removed, and the life cycle is repeated. In insect cell cultures, only the BV form of baculovirus is required, and the polyhedrin gene may be replaced with the gene for the recombinant protein. An additional benefit of replacing or deleting polyhedrin is that it effectively makes the virus unable to survive outside the laboratory, an advantage in terms of environmental safety. The system is intrinsically safe to animals, being unable to replicate in species other than a limited range of insects.

Reseña del editor

This volume focuses on the transient gene expression technologies for recombinant protein production and gene therapy. It provides coverage of an important issue in Cell Engineering by internationally authoritative experts. Topics covered range from the use of baculovirus, adenovirus, adeno-associated virus and alphavirus vectors in biopharmaceutical, genomics and gene delivery research, to large-scale transfection of plasmid DNA into cultured cells for bioreactor applications. This book illuminates all the scientific and technological issues involved in the research, development, production, and human use of viral and non-viral vectors for in vitro and in vivo expression of recombinant proteins, thus making it an essential reference for cell culture technologists, virologists, and scientists interested in gene therapy.

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Editorial
Kluwer Academic Publishers
Año de publicación
2000
Idioma
Inglés
ISBN 10 
0792365968
ISBN 13 
9780792365969
Encuadernación
Tapa dura
Número de páginas
230
Editor
Al-Rubeai Mohamed
Contacto del fabricante
ROXIO
https://www.also.com/ec/cms5/en_6000/6000/index.jsp

Friedrich-Karl-Str. 9
Hamburg
22391
Alemania

Otras ediciones populares con el mismo título

9789401058667: Cell Engineering: Transient Expression: 2

Edición Destacada

ISBN 10:  9401058660 ISBN 13:  9789401058667
Editorial: Springer, 2012
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