Pcr Topics: Usage of Polymerase Chain Reaction in Genetic and Infectious Diseases - Tapa blanda

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The Polymerase chain reaction (PCR) has revolutionized the technical development of molecular biology and immunology. It is a very simple method for amplifying nucleic acids in vitro. During the last two years PCR has mostly been used on investigations of oncogenes, tumor associated translocations, retroviral structures, HLA polymorphism and lymphokines. Because this method will make it possible to identify previously unknown structures it is particulary suitable for diagnosis in the broad field of degenerative and inflammatory diseases of the nervous system. Here we have two problems: a small amount of material and the necessity of rapid diagnostic procedures in inflammatory infections. PCR seems to be the technique which could overcome these difficulties.