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Sinopsis
Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols shares best practices collected across key laboratories and core facilities, taking the reader through key tactics for executing the most usual mass spectrometry experiments. Sections review research making use of MS proteomics experiments, focus on critical sample preparation, cover mammalian cell lines and samples from clinical tissue and biological fluids, discuss subcellular fractionation, provide methods for protein digestion both for in gel and in solution, and delve into key MS proteomics analysis protocols, including label-free LC-MS, TMT and iTRAQ labelled LC-MS, phosphorylation enrichment, ubiquination enrichment, and more. This book is the perfect lab manual for research teams or for use as a new staff training material. Core facility managers may also find it useful for sharing best practices with their staff and researchers.
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Acerca del autor
Dr. Meleady received her degree in Biotechnology from the Dublin City University and PhD from the National Cell and Tissue Culture Centre. She is currently Senior Research Scientist, Programme Leader, Proteomics Core Facility, and Associate Director National Institute for Cell Biology.
De la contraportada
Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols is the perfect reference for Bioscience researchers entering the realm of Proteomics. With a strong focus on sample preparation, the book share best practices - collected across a network of key laboratories and core facilities - taking the reader all the way to executing the most usual Mass Spectrometry experiments. In part one, the book provides a guide to extract maximum benefit from the book and an overview of proteomics including a review of exciting research making use of MS proteomics experiments. Part two focuses on the critical sample preparation stage. Chapters cover mammalian cell lines, samples from clinical tissue and biological fluids. A further chapter covers subcellular fractionation. The third part provides methods for protein digestion both for in gel and in solution, including specific methods for Shotgun proteomics. The fourth and final part of the book delves into key MS proteomics analysis protocols including label-free LC-MS, TMT and iTRAQ labelled LC-MS, phosphorylation enrichment, ubiquination enrichment, glycosylation enrichment, LC-MS methods for protein identification. The final chapter covers targeted MS using MRM. All chapters include related analytical software as appropriate. Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols is the perfect lab manual for Proteomic analysis in Biosciences. It can be used both as a reference for research teams or new staff training material. Core facility managers may find it also useful for sharing best practices with their staff and researchers.
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Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols
Librería: Revaluation Books, Exeter, Reino Unido
Calificación del vendedor: 5 de 5 estrellas
Paperback. Condición: Brand New. 350 pages. 9.25x7.50x0.55 inches. In Stock. Nº de ref. del artículo: zk0323903959
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EUR 198,85
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