Enzyme Assays: A Practical Approach: 257 (Practical Approach Series) - Tapa blanda

 
9780199638208: Enzyme Assays: A Practical Approach: 257 (Practical Approach Series)
Tapa blanda
ISBN 10: 0199638209 ISBN 13: 9780199638208
Editorial: Oxford University Press, U.S.A., 2002

Sinopsis

Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters. Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include include experimental protocols covering photometric, radiometric, H.P.L.C., and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening; statistical analysis of enzyme kinetic data; and the determination of active site concentration. This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.

"Sinopsis" puede pertenecer a otra edición de este libro.

Reseña del editor

Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters.

Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening; statistical analysis of enzyme kinetic data; and the determination of active site concentration.

This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.

Biografía del autor

Robert Eisenthal is in the Department of Biology and Biochemistry, University of Bath, U.K. . Michael Danson is in the Department of Biology and Biochemistry, University of Bath, U.K. .

"Sobre este título" puede pertenecer a otra edición de este libro.

Editorial
Oxford University Press, U.S.A.
Año de publicación
2002
Idioma
Inglés
ISBN 10 
0199638209
ISBN 13 
9780199638208
Encuadernación
Tapa blanda
Número de edición
2
Número de páginas
302
Editor
Eisenthal Robert
Contacto del fabricante
no disponible
Persona responsable
Penguin Random House Ireland Limited
productsafety@penguin.co.uk

Morrison Chambers, 32 Nassau Street
Dublin
Irlanda

Otras ediciones populares con el mismo título

9780199638215: Enzyme Assays: 257 (Practical Approach Series)

Edición Destacada

ISBN 10:  0199638217 ISBN 13:  9780199638215
Editorial: OUP Oxford, 2002
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