Sinopsis
This practical volume is designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are also described. The text goes on to explain solid-phase reagents, standards and immunogens, with procedures for their preparation. Most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.
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Reseña del editor
This practical volume is designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are also described. The text goes on to explain solid-phase reagents, standards and immunogens, with procedures for their preparation. Most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.
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Resultados de la búsqueda para Immunoassays: A Practical Approach: No.228
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Immunoassays. A Practical Approach.
Publicado por
Oxford University Press, 2000
ISBN 10: 0199637113
ISBN 13: 9780199637119
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Librería: Antiquariat Silvanus - Inhaber Johannes Schaefer, Ahrbrück, Alemania
Calificación del vendedor: 5 de 5 estrellas
304 S. mit zahlreichen Abbildungen, 0199637113 Sprache: Englisch Gewicht in Gramm: 880 4°, Original-Pappband (Hardcover), Bibliotheks-Exemplar (ordnungsgemäß entwidmet) mit Rückständen vom Rückenschild, Stempel auf der Rückseite vom Titel, insgesamt sehr gutes und innen sauberes Exemplar, Nº de ref. del artículo: 61619
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