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Sinopsis
RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly-catalytic action of the ribosome in protein synthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. This text provides a guide to the experimental procedures used in studying RNA-protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for an in-depth analysis of the structural basis of the interaction.
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RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly-catalytic action of the ribosome in protein synthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. This text provides a guide to the experimental procedures used in studying RNA-protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for an in-depth analysis of the structural basis of the interaction.
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R N A: Protein Interactions a Practical Approach
Publicado por
Oxford University Press, Oxford, 1998
ISBN 10: 0199636516
ISBN 13: 9780199636518
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Librería: Webbooks, Wigtown, Wigtown, Reino Unido
Calificación del vendedor: 5 de 5 estrellas
Hard Cover. Condición: Good. No Jacket. First Edition. Ex library with the usual blemishes. This book is heavy and will attract postal surcharges. A00003574. Nº de ref. del artículo: A00003574
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