A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques

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9780195119268: A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques
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"Makes the polymerase chain reaction (PCR) technique more accessible to researchers, physicians, and laboratory workers throughout the world. Describes the theoretical basis of the technique, practical details, and the philosophy behind the technology transfer program that the author has developed over the past 10 years. A low-cost approach is outlined for use in the study of infectious diseases, which can be applied to other technologies and applications. This approach is especially useful for laboratories in developing countries, and in high school undergraduate and continuing education programs in the US. Appendices give information on construction of equipment, and contain sample charts and worksheets. Includes b&w photos of equipment, and of men and women performing lab procedures. The author is on the faculty of the School of Public Health at UC-Berkeley."--SciTech BookNews

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The polymerase chain reaction (PCR) is a technique used to replicate specific pieces of DNA millions of times, which permits the detection and analysis of minute amounts of nucleic acids. Since its introduction in the late 1980s, this technique has been applied not only in molecular biology research but also in fields as diverse as anthropology, phylogeny, and forensics. However, despite the large impact of PCR, many of its applications remain within the confines of research and the academic environment. Now, in A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques, Dr. Eva Harris makes this elegantly simple technique more accessible to researchers, physicians, and laboratory workers throughout the world. She provides a description of the theoretical basis of the technique, the practical details of the method, and the philosophy behind the technology transfer program that she developed over the last ten years. The book serves as a guide for potential users in developing countries and for scientists in developed countries who may wish to work abroad. In addition, the low-cost approach outlined in this book can be useful for high school, undergraduate, or continuing education programs in the United States. While the specific applications of PCR outlined in the book are immediately useful to the study of infectious diseases, the approach presented can be generalized to a number of other technologies and situations. The book will help laboratories in many areas of the world generate information on site for use by physicians, epidemiologists, public health workers, and health policy professionals to develop new strategies for disease control.

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Harris, Eva
Editorial: Oxford University Press (2016)
ISBN 10: 0195119266 ISBN 13: 9780195119268
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Descripción Oxford University Press, 2016. Paperback. Estado de conservación: New. PRINT ON DEMAND Book; New; Publication Year 2016; Not Signed; Fast Shipping from the UK. No. book. Nº de ref. de la librería ria9780195119268_lsuk

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Harris, Eva
Editorial: Oxford University Press (1998)
ISBN 10: 0195119266 ISBN 13: 9780195119268
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Descripción Oxford University Press, 1998. Estado de conservación: New. Brand New, Unread Copy in Perfect Condition. A+ Customer Service! Summary: 1 Introduction2 PCR Technology2.1. Description of the Technique2.1.1. Historical Overview2.1.2. The Molecular Basis of PCR2.1.3. Advantages and Disadvantages2.2. Technical Details2.2.1. Components of the PCR Mixture2.2.2. Thermal Cycling Parameters2.2.3. Optimizing2.2.4. Potential Problems2.2.5. Detection of Products2.3. Frequently-Used PCR-Based Techniques3 Principles of Sustainable Technology Transfer3.1. A Low-Cost Methodology3.1.1. Appropriate Technology3.1.2. Alternative Techniques3.1.3. Simplification of Protocols3.1.4. In-House Preparation of Reagents3.1.5. Recycling3.1.6. Donated Materials3.2. Knowledge-Based Participatory Transfer Process3.3. Appropriate Application3.3.1. General Considerations3.3.2. An Evaluation Framework3.3.3. Assessment Criteria for PCR3.3.4. Case-by-Case Evaluation of PCR Applied to Infectious Diseases3.4. Intra-Reginal Cooperation4 Case Study: The AMB/ATT Program4.1. Introduction4.2. Program Description4.2.1. Objectives4.2.2. Format4.3. Program Structure4.3.1. Phase I4.3.2. Phase II4.3.3. Phase III4.4. Program Development4.5. Examples of Projects4.6. International CoursesII Selected Protocols5.1. PCR Protocols5.1.1. Overview5.1.2. Equipment, Materials, Control, and Procedures Common to All PCR Protocols5.1.3. Dengue Virus5.1.4. New World Leishmania5.1.5. Mycobactrium tuberculosis5.1.6. Plasmodium falciparum and Plasmodium vivax5.1.7. Vibrio cholerae5.1.8. Diarrheagenic E. coli and Shigella5.1.9. Chlamydia trachomatis and Neisseria gonorrhoeae5.1.10. Leptospira5.1.11. Trypanosoma cruzi5.2. Nonradioactive DNA Probes: V. cholerae Colony Blot5.2.1. Labeling the Probe Using PCR5.2.2. Preparation of the Colony Blot5.2.3. Hybridization5.2.4. Visualization6 Rapid Cloning of PCR Products6.1. Primer Design6.2. Preparation of PCR Products for Cloning6.2.1. Digestion of Vector and PCR Products6.2.2. Preparative Agarose Gel6.2.3. Purification of the Excised DNA Fragments Using Silica Particles6.3. Ligation6.3.1. Checking DNA Fragment Concentration by Agarose Gel Electrophoresis6.3.2. Ligation Reaction6.4. Preparation of Competent Cells and Transformation6.4.1. Preparation of Competent Cells6.4.2. Transformation6.5. Checking Clones by PCR6.6. Plasmid Purification6.7. Analysis of Clones by Restriction Enzyme Digestion6.8. Agarose Gel ElectrophoresisIII AppendixA. Construction of Laboratory EquipmentB. In-House Preparation of ReagentsB.1 Useful FormulasB.2 SolutionsB.3 Preparation of Selected ReagentsB.4 DNA Size MarkersC. Inventory for a PCR LaboratoryD. Good Laboratory PracticeD.1 General TipsD.2 Calibration of Adjustable PipettorsE. Prevention of Cross-ContaminationF. PCR Troubleshooting Guide and Flow-ChartG. Workshop Organization and Teaching TipsH. Sample Charts and WorksheetsI. Useful World Wide Web Sites. Nº de ref. de la librería ABE_book_new_0195119266

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Harris, Eva
Editorial: Oxford University Press (1998)
ISBN 10: 0195119266 ISBN 13: 9780195119268
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Irish Booksellers
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Descripción Oxford University Press, 1998. Paperback. Estado de conservación: New. book. Nº de ref. de la librería 0195119266

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Harris, Eva
Editorial: Oxford University Press
ISBN 10: 0195119266 ISBN 13: 9780195119268
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Descripción Oxford University Press. PAPERBACK. Estado de conservación: New. 0195119266 New Condition. Nº de ref. de la librería NEW4.0073646

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Harris, Eva
Editorial: Oxford University Press 1998-12 (1998)
ISBN 10: 0195119266 ISBN 13: 9780195119268
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Descripción Oxford University Press 1998-12, 1998. Estado de conservación: New. This item is printed on demand. Brand new book, sourced directly from publisher. Dispatch time is 24-48 hours from our warehouse. Book will be sent in robust, secure packaging to ensure it reaches you securely. Nº de ref. de la librería NU-LSI-06884665

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Harris, Eva
Editorial: Oxford University Press Inc, United States (1999)
ISBN 10: 0195119266 ISBN 13: 9780195119268
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Descripción Oxford University Press Inc, United States, 1999. Paperback. Estado de conservación: New. 230 x 156 mm. Language: English . Brand New Book ***** Print on Demand *****.The polymerase chain reaction (PCR) is a technique used to replicate specific pieces of DNA millions of times, which permits the detection and analysis of minute amounts of nucleic acids. Since its introduction in the late 1980s, this technique has been applied not only in molecular biology research but also in fields as diverse as anthropology, phylogeny, and forensics. However, despite the large impact of PCR, many of its applications remain within the confines of research and the academic environment. Now, in A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques, Dr. Eva Harris makes this elegantly simple technique more accessible to researchers, physicians, and laboratory workers throughout the world. She provides a description of the theoretical basis of the technique, the practical details of the method, and the philosophy behind the technology transfer program that she developed over the last ten years. The book serves as a guide for potential users in developing countries and for scientists in developed countries who may wish to work abroad. In addition, the low-cost approach outlined in this book can be useful for high school, undergraduate, or continuing education programs in the United States. While the specific applications of PCR outlined in the book are immediately useful to the study of infectious diseases, the approach presented can be generalized to a number of other technologies and situations. The book will help laboratories in many areas of the world generate information on site for use by physicians, epidemiologists, public health workers, and health policy professionals to develop new strategies for disease control. Nº de ref. de la librería AAV9780195119268

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Harris, Eva
Editorial: Oxford University Press Inc, United States (1999)
ISBN 10: 0195119266 ISBN 13: 9780195119268
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Descripción Oxford University Press Inc, United States, 1999. Paperback. Estado de conservación: New. 230 x 156 mm. Language: English . Brand New Book ***** Print on Demand *****. The polymerase chain reaction (PCR) is a technique used to replicate specific pieces of DNA millions of times, which permits the detection and analysis of minute amounts of nucleic acids. Since its introduction in the late 1980s, this technique has been applied not only in molecular biology research but also in fields as diverse as anthropology, phylogeny, and forensics. However, despite the large impact of PCR, many of its applications remain within the confines of research and the academic environment. Now, in A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques, Dr. Eva Harris makes this elegantly simple technique more accessible to researchers, physicians, and laboratory workers throughout the world. She provides a description of the theoretical basis of the technique, the practical details of the method, and the philosophy behind the technology transfer program that she developed over the last ten years. The book serves as a guide for potential users in developing countries and for scientists in developed countries who may wish to work abroad. In addition, the low-cost approach outlined in this book can be useful for high school, undergraduate, or continuing education programs in the United States. While the specific applications of PCR outlined in the book are immediately useful to the study of infectious diseases, the approach presented can be generalized to a number of other technologies and situations. The book will help laboratories in many areas of the world generate information on site for use by physicians, epidemiologists, public health workers, and health policy professionals to develop new strategies for disease control. Nº de ref. de la librería AAV9780195119268

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Harris, Eva
Editorial: OUP USA (1999)
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Descripción OUP USA, 1999. PAP. Estado de conservación: New. New Book. Shipped from US within 10 to 14 business days. THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. Nº de ref. de la librería IQ-9780195119268

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Descripción OUP USA, 1999. PAP. Estado de conservación: New. New Book. Delivered from our UK warehouse in 3 to 5 business days. THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. Nº de ref. de la librería LQ-9780195119268

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Harris, Eva
Editorial: Oxford University Press, USA (1998)
ISBN 10: 0195119266 ISBN 13: 9780195119268
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Descripción Oxford University Press, USA, 1998. Paperback. Estado de conservación: New. 1. Nº de ref. de la librería DADAX0195119266

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