Assembled in this volume are articles chosen from Methods in Enzymology Volumes 200 and 201 for their contemporary relevance and their usefulness to those new to the study of protein phosphorylation. Major topics include classification of protein kinases and protein phosphatases, assays of protein kinases and protein phosphorylation, purification and cloning of protein kinases and protein phosphatases, and the use of inhibitors of protein kinases and protein phosphatases.
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Tony Hunter received his Ph.D. in 1969 from the University of Cambridge, England. He joined the Salk Institute in 1975 as an assistant professor and has been a professor since 1982. His current interests are the protein-tyrosine kinases of the Src and growth factor receptor families and the protein-tyrosine phosphatases that remove the phosphates added by protein-tyrosine kinases. He was elected as a Fellow of the Royal Society of London in 1987, a Fellow of the American Academy of Arts and Sciences in 1992, and as an Associate Member of the European Molecular Biology Organization in 1992.
Assembled in this volume are articles from Methods in Enzymology Volumes 200 and 201 which have been chosen for their contemporary relevance and their usefulness to scientists new to the study of protein phosphorylation. It presents an overview of techniques that have defined the unexpectedly large size and degree of complexity of the protein kinase gene family.
Highlights:
* Classification of Protein Kinases and Protein Phosphatases
* Assays of Protein Kinases and Protein Phosphorylation
* Purification and Cloning of Protein Kinases and Protein Phosphatases
* Analysis and Identification of Phosphopeptides and Phosphoamino Acids
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