PCR Methods Manual: Protocols for Functional Genomics - Tapa dura

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Scientists from universities, industry, and government agencies, mostly in the US but also in Europe explain the methods for using the polyermase chain reaction technique in mRNA quantitation, diagnosis, gene discovery, genomic analysis, and expression profiling. They set out the key concepts such as thermostable DNA polymerases and optimization guidelines, and general principles of practice, then focus on specific applications. Among them are whole cell assays, comparative genomic hybridization, and the cleavase I enzyme for mutation and polymorphism scanning. The collection of articles augments rather than replaces the earlier PCR Protocols and PCR Strategies by updating theoretical notions and research experience but not duplicating the actual protocols described. Annotation c. Book News, Inc., Portland, OR (booknews.com)