The broad utility of the polymerase chain reaction (PCR) method is now within the reach of every researcher. Designed for use at the laboratory bench, this is the most comprehensive manual on PCR available today. Over 50 chapters provide precise instructions on procedures, with advice on primer design. All of the techniques described, from amplification and direct sequencing of genomic DNA through cDNA cloning and quantitation of mRNA, are tested, current, and supplemented with helpful notes and illustrations. You'll also learn how to: optimize novel applications; avoid many cumbersome molecular biological techniques; and set up the laboratory to avoid contamination. This first-rate guide will help you: avoid contamination - with specific instructions on setting up your lab; avoid cumbersome molecular biological techniques; and, discover new applications.
"Sinopsis" puede pertenecer a otra edición de este libro.
"For an up-to-date, definitive discussion of technical variations and the wide range of known applications, PCR Protocols, A Guide to Methods and Applications is an invaluable reference. This work must, by its nature, be definitive; the first three editors are from Cetus, the corporation... The editors have done well to give this work a surprisingly cohesive feel... This manual is an excellent summary of PCR to date. The index is good and the reference citations alone would save an investigator new to the field countless hours in the library. The prose and 'cookbook' presentation of the procedures are clear at an appropriate level for anyone with a background in more conventional molecular techniques. While PCR is in its infancy, this work will certainly be updated many times and by many others, however as a reflection of the scope of PCR to date, it will not be replaced or rendered obsolete."
--APPLIED CYTOGENETICS
"PCR Protocols is an excellent lab manual for making PCR work... Most of the articles are written with numbered, step-by-step procedures and have reagent information, down to the sequences of the oligonucleotides used. Even the comb binding of the book makes it suitable for bench-top use... The breadth of the applications and methodology described in this book should give even the novice the confidence and skill to make PCR work... The 30 or so applications articles give the experienced reader a good overview of the power and utility of PCR. In summary, if you have questions about PCR, PCR Protocols probably has the answers."
--TIG
"This volume owes its value to the expertise of its contributors, all specialists of international renown in the developing field of PCR....This book is an excellent compilation for all who, having a general background in molecular biology, wish to practice PCR....No user of the PCR technique could wish to miss it."
--TIBTECH
"The fifty-five chapters presented in this guide to the methods and applications of PCR provide detailed experimental protocols. They are written by researchers who use PCR successfully in their laboratory and who do not shy away from pointing out the potential pitfalls. This extremely useful publication of PCR protocols goes far beyond the simple amplification of nucleic acids... A well-organized index is provided. This book will make it more difficult to find an excuse for not using the PCR technique."
--AMERICAN JOURNAL OF PHYSIOLOGY
The broad utility of the polymerase chain reaction (PCR) method is now within the reach of every researcher. Designed for use at the laboratory bench, this is the most comprehensive manual on PCR available today. Over 50 chapters provide precise instructions on procedures, with advice on primer design. All of the techniques described, from amplification and direct sequencing of genomic DNA through cDNA cloning and quantitation of mRNA, are tested, current, and supplemented with helpful notes and illustrations. You'll also learn how to:
* Optimize novel applications
* Avoid many cumbersome molecular biological techniques
* Set up the laboratory to avoid contamination
Key Features
* This first-rate guide will help you
* Avoid contamination--with specific instructions on setting up your lab
* Avoid cumbersome molecular biological techniques
* Discover new applications
* Simply call our toll-free, 800 number listed below or cut out the coupon, fill in the blanks, and mail it to us with your check, credit-card number, or money order; We'll send you the book and you'll get your answers
"Sobre este título" puede pertenecer a otra edición de este libro.
Librería: World of Books (was SecondSale), Montgomery, IL, Estados Unidos de America
Condición: Very Good. Item in very good condition! Textbooks may not include supplemental items i.e. CDs, access codes etc. Nº de ref. del artículo: 00108782788
Cantidad disponible: 1 disponibles
Librería: World of Books (was SecondSale), Montgomery, IL, Estados Unidos de America
Condición: Good. Item in good condition. Textbooks may not include supplemental items i.e. CDs, access codes etc. Nº de ref. del artículo: 00109601857
Cantidad disponible: 1 disponibles
Librería: ThriftBooks-Dallas, Dallas, TX, Estados Unidos de America
Paperback. Condición: Good. No Jacket. Pages can have notes/highlighting. Spine may show signs of wear. ~ ThriftBooks: Read More, Spend Less. Nº de ref. del artículo: G0123721814I3N00
Cantidad disponible: 1 disponibles
Librería: ThriftBooks-Atlanta, AUSTELL, GA, Estados Unidos de America
Paperback. Condición: Good. No Jacket. Pages can have notes/highlighting. Spine may show signs of wear. ~ ThriftBooks: Read More, Spend Less. Nº de ref. del artículo: G0123721814I3N00
Cantidad disponible: 1 disponibles
Librería: ThriftBooks-Atlanta, AUSTELL, GA, Estados Unidos de America
Paperback. Condición: Very Good. No Jacket. May have limited writing in cover pages. Pages are unmarked. ~ ThriftBooks: Read More, Spend Less. Nº de ref. del artículo: G0123721814I4N00
Cantidad disponible: 1 disponibles
Librería: ThriftBooks-Dallas, Dallas, TX, Estados Unidos de America
Paperback. Condición: Fair. No Jacket. Readable copy. Pages may have considerable notes/highlighting. ~ ThriftBooks: Read More, Spend Less. Nº de ref. del artículo: G0123721814I5N00
Cantidad disponible: 1 disponibles
Librería: ThriftBooks-Dallas, Dallas, TX, Estados Unidos de America
Paperback. Condición: Very Good. No Jacket. Former library book; May have limited writing in cover pages. Pages are unmarked. ~ ThriftBooks: Read More, Spend Less. Nº de ref. del artículo: G0123721814I4N10
Cantidad disponible: 1 disponibles
Librería: -OnTimeBooks-, Phoenix, AZ, Estados Unidos de America
Condición: good. A copy that has been read, remains in good condition. All pages are intact, and the cover is intact. The spine and cover show signs of wear. Pages can include notes and highlighting and show signs of wear, and the copy can include "From the library of" labels or previous owner inscriptions. 100% GUARANTEE! Shipped with delivery confirmation, if you're not satisfied with purchase please return item! Ships via media mail. Nº de ref. del artículo: OTV.0123721814.G
Cantidad disponible: 1 disponibles
Librería: Project HOME Books, Philadelphia, PA, Estados Unidos de America
Condición: Good. shelf wear, paperback Used - Good 1990 All purchases support Project HOME - ending homelessness in Philadelphia. Nº de ref. del artículo: FK04-000076
Cantidad disponible: 1 disponibles
Librería: Better World Books Ltd, Dunfermline, Reino Unido
Condición: Good. 1st Edition. Former library copy. Pages intact with minimal writing/highlighting. The binding may be loose and creased. Dust jackets/supplements are not included. Includes library markings. Stock photo provided. Product includes identifying sticker. Better World Books: Buy Books. Do Good. Nº de ref. del artículo: 13049712-6
Cantidad disponible: 1 disponibles