The aim of this volume is to give recombinant DNA researchers the most up-to-date knowledge about heterologous gene expression in four commonly employed organisms: escherichia coli, bacillus subtilis, yeast and mammalian cells. The methods presented should enable the reader to design effective strategies for the expression of cloned genes and cDNAs, and prove useful in solving the majority of expression problems that are likely to be encountered.
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"This volume should prove invaluable to investigators with a molecular biology background who need to express a given gene but are not particularly experienced in this area. Many of the contributors have been able to effectively communicate years worth of experience and unpublished observations in what often was a black magic science. This volume clearly shows that things have changed and that the methodlolgy is now in the hands of the skilled non-expert." AMERICAN JOURNAL OF PHYSIOLOGY
The aim of this volume is to give recombinant DNA researchers the most up-to-date knowledge about heterologous gene expression in four commonly employed organisms: escherichia coli, bacillus subtilis, yeast and mammalian cells. The methods presented should enable the reader to design effective strategies for the expression of cloned genes and cDNAs, and prove useful in solving the majority of expression problems that are likely to be encountered.
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