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Proteins and Proteomics: A Laboratory Manual
Publicado por I.K. International 2005, pbk, 2005
ISBN 10: 818823737XISBN 13: 9788188237371
Libro
Contents Preface Acknowledgements Foreword 1 Introduction to Proteomics 2 One dimensional Polyacrylamide Gel Electrophoresis 3 Preparation of Cellular and Subcellular Extracts 4 Preparative Two dimensional Gel Electrophoresis with Immobilized pH Gradients 5 Reversed phase High performance Liquid Chromatography 6 Amino and Carboxy terminal Sequence Analysis 7 Peptide Mapping and Sequence Analysis of Gel resolved Proteins 8 The Use of Mass Spectrometry in Proteomics 9 Proteomic Methods for Phosphorylation Site Mapping 10 Characterization of Protein Complexes 11 Making Sense of Proteomics Using Bioinformatics to Discover a Proteins Structure Functions and Interactions 12 Appendices Reference Tables Techniques Cautions Suppliers Index The Sequencing of genomes has opened the door for exploration of the proteome the many thousands of proteins and their interactions in a given organism Reliable bench tested protocols are required for identifying isolating and quantifying proteins in a given cell or pathway and for performing functional assays Proteins and Proteomics A Laboratory Manual by Richard Simpson a leading authority in the field provides the first authoritative wide ranging technical approach to proteomics Presenting a logical strategy for analyzing proteomes Proteins and Proteomics provide background information about protein structure as well as methods for the preparation and analysis of protein samples ranging from electrophoresis and mass spectrometry to protein chips and informatics Extensive supporting information and references are given on the theory of the techniques as well as their applications This manual is an essential laboratory tool for geneticists and molecular biologists studying phenotype and cellular function and for protein chemists and biochemists seeking current techniques in this emerging field 940 pp.
