humana press apr 2004 (2 resultados)

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Protein Arrays: Methods and Protocols is an introduction to protein array technology and its application to the multiplexed detection of proteins. Although protein array technology has some roots in gene array technology, it can only be described as a distant relative. Unlike DNA, with its established rules of base pairing, and therefore predictable biochemical behavior, proteins are rich with diversity. Proteins can be large or small, compact or extended, basic or acidic, hydrophobic or hydrophilic, and so on. Just as importantly, their behavior is determined by the environment in which they reside, and so the composition of the buffer in which experiments are performed has a dramatic impact on the outcome of the experiment. Thus, if the goal is to simultaneously measure the expression of a large number of proteins, these variables must be addressed. Not to be deterred, scientists have created a variety of solutions to successfully detect and ch- acterize multiple proteins simultaneously. It is the intent of this volume to introduce to the reader a set of technological solutions to the diversity problem as well as to provide the reader with some examples of practical applications of these technologies. 300 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This collection of diverse articles by the pioneers of modern genomics takes stock of the current state of the field and elucidates the contribution that sequencing genomes has made to our understanding of microbial metabolism and evolution. Through twenty-eight thought-provoking chapters, the authors describe some of the most common computational methods and their applications to studying pathogenic microorganisms, show how genomics can be used to reconstruct the history and dynamism of the microbial world, and discuss issues as diverse as reconstruction of metabolic pathways, cell cycle processes, microbial evolution, metagenomics, and vaccine development. Additional chapters deal with microarrays and expression analysis and the role of genomic in drug discovery. Genomic technologies have transformed the field of microbiology, making it possible to investigate hundreds of genomes and uncover millions of new genes. In Microbial Genomics, a panel of genomics pioneers-many from The Institute for Genomic Research (TIGR)-take stock of the current state of the field. Through 28 thought-provoking chapters, the authors describe some of the most common computational methods and their applications to studying pathogenic microrganisms, show how genomics can be used to reconstruct the history and dynamism of the microbial world, and discuss issues as diverse as reconstruction of metabolic pathways, cell cycle processes, microbial evolution, metagenomics, and vaccine development. Additional chapters deal with microrarrays and expression analysis and the role of genomics in drug discovery. Stimulating and comprehensive, Microbial Genomics brings together not only many of the major investigators of modern microbial genomics, but also synthesizes a coherent view of the contributions that sequencing has made to our understanding of genomic structures, evolution, and biology. 556 pp. Englisch.