Primary Mesenchymal Cells: Volume V: Primary Mesenchymal Cells: 5 (Human Cell Culture) - Tapa blanda

The human body contains many specialized tissues that are capable of fulfilling an incredible variety of functions necessary for our survival. This volume in the Human Cell Culture Series focuses on mesenchymal tissues and cells. The in vitro study of mesenchymal cells is perhaps the oldest form of human cell culture, beginning with the culturing of fibroblasts. Fibroblasts have long been generically described in the literature, arising from many tissue types upon in vitro cell culture. However, recent studies, many enabled by new molecular biology techniques, have shown considerable diversity in fibroblast type and function, as described within this volume. Mesenchymal tissue types that are described within include bone, cartilage, tendons and ligaments, muscle, adipose tissue, and skin (dermis). The proper function of these tissues is predominantly dependent upon the proper proliferation, differentiation, and function of the mesenchymal cells which make up the tissue. Recent advancements in primary human mesenchymal cell culture have led to remarkable progress in the study of these tissues. Landmark experiments have now demonstrated a stem cell basis for many of these tissues, and, furthermore, significant plasticity and inter-conversion of stem cells between these tissues, resulting in a great deal of contemporary excitement and controversy. Newly-developed mesenchymal cell culture techniques have even lead to novel clinical practices for the treatment of disease.

This unique comprehensive volume in the Human Cell Culture Series encompasses research methodology for the growth and differentiation of all types of primary mesenchymal cells. Each of eleven mesenchymal cell types is covered by a leading expert in the field, providing insightful background information along with detailed current culture and assay techniques. Tissue types covered include bone, cartilage, tendons and ligaments, muscle (smooth, skeletal, and cardiac), adipose tissue, and skin (dermis), as well as multi-potent mesenchymal stem cells and fibrocytes. In addition to uses for research applications, current and future clinical applications of large-scale culture methods are also discussed. Further, the procurement and processing of primary human tissues is covered in detail. The generous inclusion of figures and tables, along with a consistent format, makes critical information highly accessible to the reader. Additionally, numerous literature citations provide a valuable reference for students and professionals in the fields of cell biology and bioengineering.