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Aldose 1-epimerase or mutarotase is the enzyme that responsible for carbohydrate metabolism and converts the alpha anomer into beta anomer of glucose. This enzyme was extracted from bovine kidney cortex. Crude enzyme exhibited the activity of 14.92 U mL-1 with specific activity of 0.153 U mg-1 proteins. The enzyme activity and specific activity was increased to 53.75 UmL-1 4.981 Umg-1 respectively after 38-60% ammonium sulfate precipitation and it was further increased to 73.27 UmL-1 and 11.67 Umg-1 when subjected to diethylaminoethyl (DEAE) cellulose chromatography. Further purification was carried out by passing it through Sephadex G-150 column and observed increase in activity 79.26 UmL-1 with 19.55 Umg-1 specific activity. The optimum pH and temperature were recorded as 8.5 and 37 oC respectively. Different stabilizers (glycerol, sodium benzoate, sodium citrate) were used to study their effect on stability of enzyme.
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Dr. Sadia Javed, PhD Biochemistry, studied at University of Agriculture, Faisalabad. Assistant Professor at Government College University, Faisalabad, Pakistan.
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- EditorialLAP LAMBERT Academic Publishing
- Año de publicación2013
- ISBN 10 365949190X
- ISBN 13 9783659491900
- EncuadernaciónTapa blanda
- Número de páginas84
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Rapid Glucose Determination by Mutarotase
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Rapid Glucose Determination by Mutarotase
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ISBN 13: 9783659491900
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Rapid Glucose Determination by Mutarotase
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Descripción Taschenbuch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Aldose 1-epimerase or mutarotase is the enzyme that responsible for carbohydrate metabolism and converts the alpha anomer into beta anomer of glucose. This enzyme was extracted from bovine kidney cortex. Crude enzyme exhibited the activity of 14.92 U mL-1 with specific activity of 0.153 U mg-1 proteins. The enzyme activity and specific activity was increased to 53.75 UmL-1 4.981 Umg-1 respectively after 38-60% ammonium sulfate precipitation and it was further increased to 73.27 UmL-1 and 11.67 Umg-1 when subjected to diethylaminoethyl (DEAE) cellulose chromatography. Further purification was carried out by passing it through Sephadex G-150 column and observed increase in activity 79.26 UmL-1 with 19.55 Umg-1 specific activity. The optimum pH and temperature were recorded as 8.5 and 37 oC respectively. Different stabilizers (glycerol, sodium benzoate, sodium citrate) were used to study their effect on stability of enzyme. 84 pp. Englisch. Nº de ref. del artículo: 9783659491900
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Rapid Glucose Determination by Mutarotase
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ISBN 10: 365949190X
ISBN 13: 9783659491900
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Descripción Taschenbuch. Condición: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Aldose 1-epimerase or mutarotase is the enzyme that responsible for carbohydrate metabolism and converts the alpha anomer into beta anomer of glucose. This enzyme was extracted from bovine kidney cortex. Crude enzyme exhibited the activity of 14.92 U mL-1 with specific activity of 0.153 U mg-1 proteins. The enzyme activity and specific activity was increased to 53.75 UmL-1 4.981 Umg-1 respectively after 38-60% ammonium sulfate precipitation and it was further increased to 73.27 UmL-1 and 11.67 Umg-1 when subjected to diethylaminoethyl (DEAE) cellulose chromatography. Further purification was carried out by passing it through Sephadex G-150 column and observed increase in activity 79.26 UmL-1 with 19.55 Umg-1 specific activity. The optimum pH and temperature were recorded as 8.5 and 37 oC respectively. Different stabilizers (glycerol, sodium benzoate, sodium citrate) were used to study their effect on stability of enzyme. Nº de ref. del artículo: 9783659491900
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Descripción PAP. Condición: New. New Book. Shipped from UK. THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. Nº de ref. del artículo: L0-9783659491900
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Descripción PAP. Condición: New. New Book. Delivered from our UK warehouse in 4 to 14 business days. THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. Nº de ref. del artículo: L0-9783659491900
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Rapid Glucose Determination by Mutarotase
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(2013)
ISBN 10: 365949190X
ISBN 13: 9783659491900
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Descripción Condición: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Javed SadiaDr. Sadia Javed, PhD Biochemistry, studied at University of Agriculture, Faisalabad. Assistant Professor at Government College University, Faisalabad, Pakistan.Aldose 1-epimerase or mutarotase is the enzyme that respon. Nº de ref. del artículo: 5159658
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