Enzymes: Biochemistry, Biotechnology, Clinical Chemistry: Biochemistry, Biotechnology and Clinical Chemistry - Tapa blanda

Review of the first edition: "Essential. ...explains the structure and function of enzymes. ...every chapter tells of an advance in understanding proteins or their interactions. I will definitely use this book." --Chemistry and Industry (Professor Carole Hackney, Keele University, UK)

"Outstandingly written, updated on molecular biology and bioinformatics, with particularly good treatment of steady-state enzyme kinetics and analytical applications. For undergraduates in biochemistry, biological and analytical sciences, and medicine." --Natural Products Report (Royal Society of Chemistry)

"An excellent book, warmly recommended. --Trends in Biotechnology

In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course.

• Provides an introduction to enzymology and a balanced account of the theoretical and applied aspects of the subject
• Discusses techniques such as membrane chromatography, aqueous phase partitioning and engineering recombinant proteins for purification
• Includes a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy