Protein Expression in Animal Cells (v. 43) (Methods in Cell Biology)

Protein Expression in Animal Cells presents information essential to researchers and students investigating cellular function. This exceptional laboratory guide covers the selection and use of expression systems that are optimal for specific exogenous proteins. Current studies often require protein expression in particular cell types, in amounts suitable for specific detection methods, and under conditions that do not otherwise perturb the specific cellular mechanisms under investigation. Such demanding requirements imply tht there is no universal vector for all experiments. This comprehensive manual describes the structure, production, host range, and practical use of diverse viral and plasmid vectors. Protocols are written at a level geared to the beginning lab student. Because it covers many versatile expression systems, expert researchers will also find this guidebook a valuable addition to their laboratory.