Molecular Genetic Analysis of Populations: A Practical Approach: No. 187. - Tapa dura

Methods enabling the direct study of genetic variation in natural populations have improved considerably. This new edition explores these updated techniques in DNA analysis. It provides a revised and refined guide to investigating variation in DNA molecules. Contents: Starch gel electrophoresis of allozymes; Total DNA isolation; mtDNA isolation, Separation and detection of fragments; Genomic libraries and the development of species-specific probes; RFLP analysis using heterologous probes; PCR protocols and population analysis by direct DNA sequencing and PCR-based DNA fingerprinting; Microsatellites; Detection of genetic variation by DNA conformational and denaturing gradient methods; Multi- and single-locus DNA fingerprinting; Automated DNA detection with fluorescence-based technologies; Appendix: List of Suppliers; Index. This book is intended for postgraduate and postdoctoral researchers, academics, and clinical staff.