Analysis of lycopene in tomato (Lycopersicon esculentum, Mill.) fruits: Determination of lycopene in tomato (Lycopersicon esculentum, Mill.) by HPLC & Vis-NIR spectroscopy

ISBN 10: 3847327488 / ISBN 13: 9783847327486
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Analysis of lycopene in tomato (Lycopersicon esculentum, Mill.) fruits: Determination of lycopene in tomato (Lycopersicon esculentum, Mill.) by HPLC & Vis-NIR spectroscopy. N° de ref. de la librería

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Sinopsis: Lycopene is highly unsaturated, open-chain carotenoid found abundantly in tomatoes. Research interests into properties & health benefits of lycopene have been growing, bringing attention to content of lycopene in food. The purpose of this work was to develop efficient & accurate protocol that can measure lycopene using HPLC, and establish relationship with Vis/NIR reflectance spectroscopy measurement. Accordingly, mobile phase composition ACN/MeOH (50:50, v/v)+ TEA 9 µM; extraction solvents hexane/acetone/ethanol (50:25:25, v/v/v); re-dissolving residue in THF, followed ACN/MeOH (15:30:55, v/v/v); flow rate 0.6 mL/min and ?detection 472 nm were showed most suitable for lycopene determination in tomato. This method has achieved best characteristic spectra profile with precision (RSD > 15), accuracy & recovery (? 81.7%) and sensitive detection limit (0.0156 ?g/mL) within separation of ~ 21 minutes. Finally, the established method was applied to selected tomato cultivars and a wide range of lycopene content 1.91 to 8.23 mg/100g was observed. Besides, although HPLC is most accepted method; Vis/NIR combined with multivariate techniques has showed potential for determining lycopene.

Biografía del autor: Mr. Wondu Garoma has obtained Diploma in plant Science in 1997, BSc Degree in Biology in 2004, & European Master of Science Degree in Food Science, Technology & Nutrition in 2008. Since then he has worked in different higher institutions. Currently, He is lecturer & Dean at Food & Bio-process Technology Institute, Wollega University,Ethiopia.

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Título: Analysis of lycopene in tomato (Lycopersicon...
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Berra, Wondu Garoma
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Descripción LAP Lambert Academic Publishing, Germany, 2011. Paperback. Estado de conservación: New. Aufl.. 220 x 150 mm. Language: English . Brand New Book ***** Print on Demand *****.Lycopene is highly unsaturated, open-chain carotenoid found abundantly in tomatoes. Research interests into properties health benefits of lycopene have been growing, bringing attention to content of lycopene in food. The purpose of this work was to develop efficient accurate protocol that can measure lycopene using HPLC, and establish relationship with Vis/NIR reflectance spectroscopy measurement. Accordingly, mobile phase composition ACN/MeOH (50:50, v/v)+ TEA 9 M; extraction solvents hexane/acetone/ethanol (50:25:25, v/v/v); re-dissolving residue in THF, followed ACN/MeOH (15:30:55, v/v/v); flow rate 0.6 mL/min and detection 472 nm were showed most suitable for lycopene determination in tomato. This method has achieved best characteristic spectra profile with precision (RSD > 15), accuracy recovery ( 81.7 ) and sensitive detection limit (0.0156 g/mL) within separation of 21 minutes. Finally, the established method was applied to selected tomato cultivars and a wide range of lycopene content 1.91 to 8.23 mg/100g was observed. Besides, although HPLC is most accepted method; Vis/NIR combined with multivariate techniques has showed potential for determining lycopene. Nº de ref. de la librería AAV9783847327486

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Descripción LAP Lambert Academic Publishing. Paperback. Estado de conservación: New. Paperback. 112 pages. Dimensions: 8.7in. x 5.9in. x 0.3in.Lycopene is highly unsaturated, open-chain carotenoid found abundantly in tomatoes. Research interests into properties and health benefits of lycopene have been growing, bringing attention to content of lycopene in food. The purpose of this work was to develop efficient and accurate protocol that can measure lycopene using HPLC, and establish relationship with VisNIR reflectance spectroscopy measurement. Accordingly, mobile phase composition ACNMeOH (50: 50, vv) TEA 9 M; extraction solvents hexaneacetoneethanol (50: 25: 25, vvv); re-dissolving residue in THF, followed ACNMeOH (15: 30: 55, vvv); flow rate 0. 6 mLmin and detection 472 nm were showed most suitable for lycopene determination in tomato. This method has achieved best characteristic spectra profile with precision (RSD 15), accuracy and recovery ( 81. 7) and sensitive detection limit (0. 0156 gmL) within separation of 21 minutes. Finally, the established method was applied to selected tomato cultivars and a wide range of lycopene content 1. 91 to 8. 23 mg100g was observed. Besides, although HPLC is most accepted method; VisNIR combined with multivariate techniques has showed potential for determining lycopene. This item ships from multiple locations. Your book may arrive from Roseburg,OR, La Vergne,TN. Paperback. Nº de ref. de la librería 9783847327486

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