Somatic Cell Nuclear Transfer in Goat (Capra hircus)

 
9783848426546: Somatic Cell Nuclear Transfer in Goat (Capra hircus)
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Nuclear transfer is a very effective method for propagation of valuable, extinct and endangered animals. Hand-made cloning (HMC) is an effective alternative to the conventional micromanipulator based technique in some domestic species. The present study was carried out for the establishment of an effective enucleation method, for selection of suitable somatic cell as nuclear donor and for development of an optimum culture system for in vitro culture of zona free cloned embryos of goat (Capra hircus). An efficient chemically assisted enucleation procedure was established for goat HMC. Treatment of donor cells with roscovitine resulted in better hand-made cloned blastocyst yield. Production of hand-made cloned goat embryos was better with electric pulse activation than Ca ionophore activation. A highly effective culture system for the development of hand-made cloned goat embryos was established. Furthermore, fetal fibroblast cells proved to be better nuclear donor than fresh cumulus cells or cultured cumulus cells.

Biografía del autor:

Yogesh S. Akshey, Arun Kumar De and Dhruba Malakar:Currently working on in vitro and in vivo production of embryos and their transfer to recipient animals, stem cells, somatic cell nuclear transfer, induced pluripotent stem cells and regulation of oocyte competence in farm animals. Published a number of research articles in reputed journals.

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Yogesh S Akshey
Publicado por LAP Lambert Academic Publishing 2012-03-02 (2012)
ISBN 10: 3848426544 ISBN 13: 9783848426546
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Akshey, Yogesh S. / De, Arun K.
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Descripción Condición: New. Publisher/Verlag: LAP Lambert Academic Publishing | Production of Cloned Embryos by Hand-made Cloning Method | Nuclear transfer is a very effective method for propagation of valuable, extinct and endangered animals. Hand-made cloning (HMC) is an effective alternative to the conventional micromanipulator based technique in some domestic species. The present study was carried out for the establishment of an effective enucleation method, for selection of suitable somatic cell as nuclear donor and for development of an optimum culture system for in vitro culture of zona free cloned embryos of goat (Capra hircus). An efficient chemically assisted enucleation procedure was established for goat HMC. Treatment of donor cells with roscovitine resulted in better hand-made cloned blastocyst yield. Production of hand-made cloned goat embryos was better with electric pulse activation than Ca ionophore activation. A highly effective culture system for the development of hand-made cloned goat embryos was established. Furthermore, fetal fibroblast cells proved to be better nuclear donor than fresh cumulus cells or cultured cumulus cells. | Format: Paperback | Language/Sprache: english | 128 pp. Nº de ref. del artículo: K9783848426546

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Yogesh S Akshey
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Descripción LAP Lambert Academic Publishing, 2012. PAP. Condición: New. New Book. Delivered from our UK warehouse in 3 to 5 business days. THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. Nº de ref. del artículo: LQ-9783848426546

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Akshey, Yogesh S.
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Descripción LAP Lambert Academic Publishing, 2016. Paperback. Condición: New. PRINT ON DEMAND Book; New; Publication Year 2016; Not Signed; Fast Shipping from the UK. No. book. Nº de ref. del artículo: ria9783848426546_lsuk

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Yogesh S. Akshey
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Descripción LAP Lambert Academic Publishing Mrz 2012, 2012. Taschenbuch. Condición: Neu. This item is printed on demand - Print on Demand Neuware - Nuclear transfer is a very effective method for propagation of valuable, extinct and endangered animals. Hand-made cloning (HMC) is an effective alternative to the conventional micromanipulator based technique in some domestic species. The present study was carried out for the establishment of an effective enucleation method, for selection of suitable somatic cell as nuclear donor and for development of an optimum culture system for in vitro culture of zona free cloned embryos of goat (Capra hircus). An efficient chemically assisted enucleation procedure was established for goat HMC. Treatment of donor cells with roscovitine resulted in better hand-made cloned blastocyst yield. Production of hand-made cloned goat embryos was better with electric pulse activation than Ca ionophore activation. A highly effective culture system for the development of hand-made cloned goat embryos was established. Furthermore, fetal fibroblast cells proved to be better nuclear donor than fresh cumulus cells or cultured cumulus cells. 128 pp. Englisch. Nº de ref. del artículo: 9783848426546

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Yogesh S. Akshey
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Descripción LAP Lambert Academic Publishing Mrz 2012, 2012. Taschenbuch. Condición: Neu. Neuware - Nuclear transfer is a very effective method for propagation of valuable, extinct and endangered animals. Hand-made cloning (HMC) is an effective alternative to the conventional micromanipulator based technique in some domestic species. The present study was carried out for the establishment of an effective enucleation method, for selection of suitable somatic cell as nuclear donor and for development of an optimum culture system for in vitro culture of zona free cloned embryos of goat (Capra hircus). An efficient chemically assisted enucleation procedure was established for goat HMC. Treatment of donor cells with roscovitine resulted in better hand-made cloned blastocyst yield. Production of hand-made cloned goat embryos was better with electric pulse activation than Ca ionophore activation. A highly effective culture system for the development of hand-made cloned goat embryos was established. Furthermore, fetal fibroblast cells proved to be better nuclear donor than fresh cumulus cells or cultured cumulus cells. 128 pp. Englisch. Nº de ref. del artículo: 9783848426546

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Yogesh S. Akshey
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Descripción LAP Lambert Academic Publishing Mrz 2012, 2012. Taschenbuch. Condición: Neu. Neuware - Nuclear transfer is a very effective method for propagation of valuable, extinct and endangered animals. Hand-made cloning (HMC) is an effective alternative to the conventional micromanipulator based technique in some domestic species. The present study was carried out for the establishment of an effective enucleation method, for selection of suitable somatic cell as nuclear donor and for development of an optimum culture system for in vitro culture of zona free cloned embryos of goat (Capra hircus). An efficient chemically assisted enucleation procedure was established for goat HMC. Treatment of donor cells with roscovitine resulted in better hand-made cloned blastocyst yield. Production of hand-made cloned goat embryos was better with electric pulse activation than Ca ionophore activation. A highly effective culture system for the development of hand-made cloned goat embryos was established. Furthermore, fetal fibroblast cells proved to be better nuclear donor than fresh cumulus cells or cultured cumulus cells. 128 pp. Englisch. Nº de ref. del artículo: 9783848426546

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Akshey, Yogesh S.
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Descripción LAP Lambert Academic Publishing, 2012. PAP. Condición: New. New Book. Shipped from US within 10 to 14 business days. THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. Nº de ref. del artículo: IQ-9783848426546

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Yogesh S Akshey, Arun Kumar De, Dhruba Malakar
Publicado por LAP Lambert Academic Publishing, Germany (2012)
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Descripción LAP Lambert Academic Publishing, Germany, 2012. Paperback. Condición: New. Aufl.. Language: English . Brand New Book. Nuclear transfer is a very effective method for propagation of valuable, extinct and endangered animals. Hand-made cloning (HMC) is an effective alternative to the conventional micromanipulator based technique in some domestic species. The present study was carried out for the establishment of an effective enucleation method, for selection of suitable somatic cell as nuclear donor and for development of an optimum culture system for in vitro culture of zona free cloned embryos of goat (Capra hircus). An efficient chemically assisted enucleation procedure was established for goat HMC. Treatment of donor cells with roscovitine resulted in better hand-made cloned blastocyst yield. Production of hand-made cloned goat embryos was better with electric pulse activation than Ca ionophore activation. A highly effective culture system for the development of hand-made cloned goat embryos was established. Furthermore, fetal fibroblast cells proved to be better nuclear donor than fresh cumulus cells or cultured cumulus cells. Nº de ref. del artículo: KNV9783848426546

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Akshey, Yogesh S.
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Descripción LAP LAMBERT Academic Publishing. Condición: New. Paperback. Worldwide shipping. FREE fast shipping inside USA (express 2-3 day delivery also available). Tracking service included. Ships from United States of America. Nº de ref. del artículo: 3848426544

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