<P>IN <I>DNA CLONING AND ASSEMBLY METHODS,</I> EXPERT RESEARCHERS IN THE FIELD DETAIL MANY OF THE METHODS WHICH ARE NOW COMMONLY USED FOR DNA CLONING AND MAKE CLONING PROCEDURES FASTER, MORE RELIABLE AND ALSO SUITABLE FOR HIGH-THROUGHPUT HANDLING. THESE INCLUDE METHODS AND PROTOCOLS THAT ARE BASED ON SEVERAL MECHANISMS INCLUDING TYPE II AND IIS RESTRICTION ENZYMES, SINGLE STRANDED ANNEALING, SEQUENCE OVERLAP, AND RECOMBINATION.</P><P>WITH ADDITIONAL CHAPTERS ON SOFTWARE PROGRAMS THAT ARE SUITABLE FOR PRIMER DESIGN, A FEATURE CRUCIAL FOR THE FUNCTIONALITY OF THE DESCRIBED METHODS. WRITTEN IN THE HIGHLY SUCCESSFUL <I>METHODS IN MOLECULAR BIOLOGY </I>SERIES FORMAT, CHAPTERS INCLUDE INTRODUCTIONS TO THEIR RESPECTIVE TOPICS, LISTS OF THE NECESSARY MATERIALS AND REAGENTS, STEP-BY-STEP, READILY REPRODUCIBLE LABORATORY PROTOCOLS, AND KEY TIPS ON TROUBLESHOOTING AND AVOIDING KNOWN PITFALLS.</P><P>AUTHORITATIVE AND PRACTICAL, <I>DNA CLONING AND ASSEMBLY METHODS</I> SEEKS TO PROVIDE SCIENTIST WITH A VALUABLE AND USEFUL RESOURCE FOR WET LAB RESEARCHERS WITHIN LIFE SCIENCES.</P>
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In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination.
With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide scientist with a valuable and useful resource for wet lab researchers within life sciences.
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Condición: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes methods based on mechanismsProvides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the expertsIn DNA Cloning and Assembly Methods, expert researchers in th. Nº de ref. del artículo: 4267729
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination.With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide scientist with a valuable and useful resource for wet lab researchers within life sciences. 316 pp. Englisch. Nº de ref. del artículo: 9781627037631
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