HPLC of Peptides and Proteins: Methods and Protocols (Methods in Molecular Biology)

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9780896039773: HPLC of Peptides and Proteins: Methods and Protocols (Methods in Molecular Biology)

The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.

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From the Back Cover:

High performance liquid chromatography (HPLC) plays a critical role today in both our understanding of biological processes and in the development of peptide and protein-based pharmaceuticals. In HPLC of Peptides and Proteins: Methods and Protocols, leading experts from academia and industry comprehensively describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation. The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology. These applications include proteolytic mapping, posttranslational modification, neuropeptide processing, glycopeptides and glycoproteins, MHC-binding peptides, toxins/venoms, membrane proteins, antibodies, combinatorial and proteome analysis, and enzymatic activity. Each readily reproducible protocol contains background notes, step-by-step instructions, reagent and equipment lists, and tips on both troubleshooting and avoiding known pitfalls.
Comprehensive and easy-to-follow, HPLC of Peptides and Proteins: Methods and Protocols offers every biologist and biotechnologist at all levels of expertise ready access to the powerful tools needed to develop and carry out peptide and protein separations and analyses today.

Review:

"...a showcase of state-of-the-art methodologies in the application of HPLC to protein/peptide studies...can be used by beginners and intermediate experts in this field to develop separation and analytical protocols...all scientists dealing with peptides and proteins can benefit from reading this book." - Proteomics

"...this book is well written...a very usable laboratory companion or reference guide for researchers in pursuit of peptide and protein characterizations." - Biomedical Chromatography

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Editor-Marie-Isabel Aguilar
Editorial: Humana Press (2003)
ISBN 10: 0896039773 ISBN 13: 9780896039773
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Descripción Humana Press, 2003. Hardcover. Estado de conservación: New. Nº de ref. de la librería SONG0896039773

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Marie-Isabel Aguilar
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Descripción Humana Press Inc., United States, 2004. Hardback. Estado de conservación: New. 2004 ed.. Language: English . This book usually ship within 10-15 business days and we will endeavor to dispatch orders quicker than this where possible. Brand New Book. The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age.The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins. Nº de ref. de la librería LIE9780896039773

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Editorial: Humana Press (2003)
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Descripción Humana Press Inc., 2003. HRD. Estado de conservación: New. New Book. Delivered from our US warehouse in 10 to 14 business days. THIS BOOK IS PRINTED ON DEMAND.Established seller since 2000. Nº de ref. de la librería IP-9780896039773

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MARIE-ISABEL AGUILAR
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Descripción Humana Press, 2003. Hardback. Estado de conservación: NEW. 9780896039773 This listing is a new book, a title currently in-print which we order directly and immediately from the publisher. Nº de ref. de la librería HTANDREE0287294

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Marie-Isabel Aguilar (Editor)
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Descripción Humana Press, 2003. Hardcover. Estado de conservación: New. 2004. Nº de ref. de la librería DADAX0896039773

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Marie-Isabel Aguilar
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Descripción Humana Press Inc., United States, 2004. Hardback. Estado de conservación: New. 2004 ed.. Language: English . Brand New Book ***** Print on Demand *****.The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age.The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins. Nº de ref. de la librería APC9780896039773

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Marie-Isabel Aguilar
Editorial: Humana Press Inc., United States (2004)
ISBN 10: 0896039773 ISBN 13: 9780896039773
Nuevos Tapa dura Cantidad: 10
Impresión bajo demanda
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(London, Reino Unido)
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Descripción Humana Press Inc., United States, 2004. Hardback. Estado de conservación: New. 2004 ed.. Language: English . Brand New Book ***** Print on Demand *****. The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age.The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins. Nº de ref. de la librería APC9780896039773

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