Imaging: A Laboratory Manual - Tapa blanda

Imaging has Rapidly Become a very Valuable approach in nearly every field of biological research. Finding the right method and optimizing it for data collection can be daunting, even for an established imaging laboratory. Imaging: A Laboratory Manual is the cornerstone of a new laboratory manual series, designed as an essential guide for investigators who need these visualization techniques. Written by leading imaging experts, this first volume is meant as a general reference for all fields and describes the theory and practice of a wide array of methods.

Imaging: A Laboratory Manual is organized into three sections, each containing background information and step-by-step protocols.

Instrumentation: Chapters in this section focus on imaging hardware and the basics of light microscopy, light sources, cameras, and image processing. Different forms of imaging---from DIC to confocal to two-photon microscopy---are discussed in detail. Novel technologies like liquid crystals, acousto-optical tunable filters, ultrafast lasers, and grating systems are included, as is a chapter on making the microscope environment compatible with the survival of common biological preparations.

Labeling and Indicators: Chapters in this section provide detailed instructions on labeling methods to stain cells, organelles, and proteins as well as to measure ions and detect molecular interactions. Subjects range from well-established methods such as immunological and nonimmuno-logical staining to newer genetic engineering techniques. The section also discusses fluorescent and luminescent indicators of several intracellular biochemical pathways, with a particular emphasis on measurements of calcium dynamics.

Advanced Microscopy: Chapters in this section discuss newer techniques at the cutting edge of imaging research. The section is divided by scale, with the first chapters covering imaging at the molecular level. Molecular approaches include FRET, FIONA, PALM, STORM, quantum dots, and AFM. The next set of chapters looks at imaging on a cellular level; techniques include TIRFM, fluorescence correlation spectroscopy, image correlation spectroscopy, FLIM, FRAP, fluorescence speckle microscopy, polarized light microscopy, array tomography, and second-harmonic generation. Whole-organism imaging is also covered, with methods that include coherent Raman tissue imaging, light-sheet-based fluorescent microscopy, SPIM, microendoscopy, and photo-acoustic imaging. The section closes with a series of chapters on fast imaging and the use of caged compounds.

The manual also includes a set of appendices with a glossary of imaging terms and other useful information on spectra, lenses, filters, and the safe handling of imaging equipment.

From the basic chapters on optics to the protocols for advanced, specialized methods, Imaging: A Laboratory Manual provides a comprehensive resource vital to the modern biology laboratory.

In recent years, imaging has rapidly become a tremendously valuable approach in nearly every field of biological research. Finding the right method and optimizing it for data collection can be a daunting process, even for an established imaging laboratory. Imaging: A Laboratory Manual is the cornerstone of a new laboratory manual series, designed as an essential guide for investigators who need these visualization techniques. This first volume is meant as a general reference for all fields, and describes the theory and practice of a wide array of imaging methods. From the basic chapters on optics, equipment and labeling to detailed explanations of advanced, cutting-edge methods like PALM, STORM, light sheet and high speed microscopy, Imaging: A Laboratory Manual is a vital resource for the modern biology laboratory.