Purifying Proteins for Proteomics: A Laboratory Manual - Tapa blanda

This substantial text provides researchers with detailed descriptions of laboratory methods used to prepare proteins for proteomic analysis using chromatography and electrophoresis. Six chapters are also included for the analysis of purified proteins prior to structural proteomics, with topics including analytical ultracentrifugation, measuring conformational stability, protein characterization with mass spectrometry, and analyzing carbohydrate from glycoproteins. Each chapter is clearly organized in outline form and includes discussion, methods, troubleshooting, and protocols. Appendices are provided that detail protein structure, measuring concentration of proteins, concentrating solutions, protein stabilization for storage, one-dimensional polyacrylamide gel electrophoresis, and troubleshooting guides and cautions. The manual will accompany Proteins and proteomics by the same author. Simpson, who authored many of the chapters as well as editing the text, is at the Joint ProteomicS Laboratory (JPSL) of the Ludwig Institute for Cancer Research in Melbourne, Australia. Annotation ©2004 Book News, Inc., Portland, OR (booknews.com)

This manual complements Simpson's Proteins and Proteomics manual, with a comprehensive collection of methods for protein purification from a variety of source preparations. The chapters include detailed protocols, methods for optimizing the performance of experiments, discussion of potential pitfalls, and troubleshooting advice.