Proteins and Proteonics : a Laboratory Manual - Tapa blanda

Describes the principles, analytical methods, and protocols involved in the study of proteomics, covering the separation of proteins, liquid chromatography, and sequence analysis.

... the proteome is multidimensional: a single gene can specify a number of different protein products that may themselves be modified post-translationally by the covalent attachment of a range of functional groups. Moreover, proteins commonly occur in complexes whose composition and conformation have a profound effect on both their activity and their stability. Proteomics attempts to grapple with this complexity by using a vast range of molecular, chemical and genetic techniques. This superb volume embraces this diversity and, even if proteomic techniques are far from comprehensive in their scope, this weighty tome is certainly comprehensive in its description of them. Everything is here, from detailed laboratory protocols to clear explanations of the theoretical basis of the methods used. The book is as up-to-date as possible. It is peppered with references from 2002, and deals fully with recent technical advances, such as protein microarrays. Richard Simpson even manages to slip in bits of history, such as the derivation of the name Coomassie for the blue dye used to stain proteins in gels. (It was named to commemorate the British conquest of Kumasie-the capital of the Ashanti, in what is now Ghana-and was originally used to dye woollen jumpers.) Such nuggests make this volume more than just an excellent laboratory manual; it is also a book to dip into and enjoy. Nature Proteins and Proteomics: A Laboratory Manual is an invaluable information tool both for the experienced protein chemist who bravely ventures into the new world of proteomics and for the novice to proteins and proteomes. By focusing on what is currently considered the bedrock of proteomics technologies, Professor Simpson ensures that - in spite of the rapid advances that characterize contemporary proteomics research - this volume will remain relevant and current for years to come. -- From the Preface by Ruedi Aebersold